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Expanding the targeting scope of FokI-dCas nuclease systems with SpRY and Mb2Cas12a.
Cheng Y, Sretenovic S, Zhang Y, Pan C, Huang J, Qi Y
Biotechnol J. 2022 Apr 4:e2100571. doi: 10.1002/biot.202100571. PubMed Article

Plasmids from Article

ID Plasmid Purpose
177681pYPQ-NF1-L1-dSpRYGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing
177682pYPQ-NF1-L2-dSpRYGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing
177683pYPQ-NF-L1-dMb2Cas12aGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing
177684pYPQ-NF-L2-dMb2Cas12aGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing
177686pYPQ-NF-L3-NF-L1-dMb2Cas12aGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

Antibodies from Article