Real-Time Monitoring of the Yeast Intracellular State During Bioprocesses With a Toolbox of Biosensors.
Torello Pianale L, Rugbjerg P, Olsson L
Front Microbiol. 2022 Jan 7;12:802169. doi: 10.3389/fmicb.2021.802169. eCollection 2021. PubMed Article
Torello Pianale L, Rugbjerg P, Olsson L
Front Microbiol. 2022 Jan 7;12:802169. doi: 10.3389/fmicb.2021.802169. eCollection 2021. PubMed Article
A subset of these plasmids is included in the Olsson - ScEnSor Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177705 | YN2_1_LT58_X2site | Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae |
| 177706 | LT1_33_pTEFmut8-mCherry | Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter |
| 177707 | LT1_34_sfpHluorin | Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor) |
| 177708 | LT1_36_QUEEN-2m | Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor) |
| 177709 | LT2_7_OxPro | Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor) |
| 177710 | LT2_12_GlyRNA | Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor) |
| 177711 | LT2_14_GlyOx | Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress) |
| 177712 | YN2_1_LT84_RPL13A | Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae |