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ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function.
Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC
Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
177715pPLN-hFluc-WT-KDELFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention
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177716pMTS-hFlucFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting
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177717pAT-WT-MycER protein alpha-1-antitrypsin (WT), myc tagged
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177718pATZ-MycER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged
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177719pAT-WT-HA-GFP11ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP
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177720pATZ-HA-GFP11ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP
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177721pATZ-BFP-HA-GFP11ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP
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177722pPLN-hFluc-WT-HA-GFP11-KDELFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
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177723pPLN-hFluc-DM-HA-GFP11-KDELDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
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177724pGal-Trans-Myc-HA-GFP11-KDELER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP
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177725pMTS-HA-GFP11Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged
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177726pMTS-hFluc-HA-GFP11Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminus
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177727pPLN-hFluc-WT-eGFP-KDELFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus
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177728pPLN-hFluc-DM-eGFP-KDELDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus
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177729pGal-Trans-Myc-eGFP-KDELER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged
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177730p403 – TDH3-MTS-mCherry-GFP1-10-His mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker
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177731p406 – TDH3-ER-FlucDM-GFP11-Ura DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with
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177732p406 – TDH3-ER-FlucDM-GFP- Ura DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker
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177733p406 – TDH3-MTS-GFP11-Ura Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA marker
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177734p406 – TDH3-Elo3-GFP11-Ura ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker
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Recombinant Antibodies from Article