NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens.
Stukenberg D, Hoff J, Faber A, Becker A
Commun Biol. 2022 Mar 25;5(1):265. doi: 10.1038/s42003-022-03150-0. PubMed Article
Stukenberg D, Hoff J, Faber A, Becker A
Commun Biol. 2022 Mar 25;5(1):265. doi: 10.1038/s42003-022-03150-0. PubMed Article
These plasmids are available as a kit: NT-CRISPR Plasmid Collection.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179332 | pST_116_LVL2 cam | NT-CRISPR plasmid for a single gRNA. |
| 179333 | pST_119_LVL2 cam | NT-CRISPR plasmid for integration of multiple gRNAs. |
| 179334 | pST_140_LVL2 cam | NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant). |
| 179335 | pMC0x_gRNA_Pos4_Ptet | gRNA Position Vector 4, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam |
| 179336 | pMC0x_gRNA_Pos5_Ptet | gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam |
| 179337 | pMC0x_gRNA_Pos5a_Ptet | gRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam |
| 179338 | pMC0x_gRNA_Pos5b_Ptet | gRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam |
| 179339 | pMC0x_gRNA_Pos5a1_Ptet | gRNA Position Vector 5a1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam |
| 179340 | pMC0x_gRNA_Pos5a2_Ptet | gRNA Position Vector 5a2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam |
| 179341 | pMC0x_gRNA_Pos5b1_Ptet | gRNA Position Vector 5b1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam |
| 179342 | pMC0x_gRNA_Pos5b2_Ptet | gRNA Position Vector 5b2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam |