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In vivo proximity biotin ligation identifies the interactome of Egalitarian, a Dynein cargo adaptor.
Baker FC, Neiswender H, Veeranan-Karmegam R, Gonsalvez GB
Development. 2021 Nov 15;148(22). pii: 273472. doi: 10.1242/dev.199935. Epub 2021 Nov 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
179993pUASp-Egl-TrboExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.
179995pUASp-GBP-TbroExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.
179996pUASt-GBP-TrboExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.

Antibodies from Article