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Addgene
A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells
Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. Epub 2019 Nov 5. PubMed Article

These plasmids are not included in The Mammalian Toolkit. To order the Mammalian Toolkit, please see the following kit page.

Plasmids from Article

ID Plasmid Purpose
123952MTK3_001Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system
123953MTK3b_023Encodes human RelA as a Type 3b part to be used in the MTK system
123954MTK3b_025Encodes synTF fused to VP16 activation domain as a Type 3b part to be used in the MTK system
123955MTK3b_026Encodes synTF fused to VP64 activation domain as a Type 3b part to be used in the MTK system
123956MTK4a_009Encodes a nuclear localized EBFP2 (T2A) coexpression cassette as a Type 4a part to be used in the MTK system
123957MTK234_009Encodes the spCas9 pC120 gRNA expression cassette as a type 234 part to be used in the MTK system
123958MTK234_011Encodes the spCas9 pPLMP1 gRNA expression cassette as a type 234 part to be used in the MTK system
123959MTK234_054Encodes the saCas9 CXCR1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123960MTK0_002Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system
123961MTK0_004Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
123964MTK0_009Encodes PhiC31 landing pad as a type 0 part to be used in the MTK system
123965MTK0_010Encodes BxBI landing pad as a type 0 part to be used in the MTK system
123966MTK0_011Encodes the hROSA26 PhiC31 recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
123967MTK0_012Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
123968MTK0_028Encodes the cROSA26 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
123969MTK0_030Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system
123970MTK0_031Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system
123971MTK0_033Encodes the cROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK system
123972MTK0_034Encodes the hROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK system
123973MTK0_036Encodes the cROSA26 BxBI landing pad as a type 0 part to be used in the MTK system
123974MTK0_037Encodes the hROSA26 BxBI landing pad as a type 0 part to be used in the MTK system
123975MTK0_045Encodes the cROSA26 destination with hygyomycin resistance cassette as a type 0 part to be used in the MTK system
123976MTK0_046Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh
123977MTK0_047Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
123978MTK0_052Encodes the PhiC31 landing pad on hThal5.10-2-hg18 with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
123980MTK0_054Encodes the PhiC31 landing pad on hCLYBL with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
123982MTK234_057Encodes the spCas9 Safetargeting Chr20 gRNA expression cassette as a type 234 part to be used in the MTK system
123984MTK234_058Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system
123988MTK234_012Encodes the spCas9 sgRNA1 hROSA26 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123989MTK234_013Encodes the spCas9 sgRNA1 hROSA26 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123990MTK234_014Encodes the spCas9 sgRNA1 hROSA26 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123991MTK234_015Encodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system
123992MTK234_005Encodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123993MTK234_006Encodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123994MTK234_007Encodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123995MTK234_032Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123996MTK234_033Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123997MTK234_034Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123998JPF0507Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
123999JPF0508Encodes pCAG driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124000JPF0509Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124001JPF0510Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124002JPF0511Encodes pHUBC driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124003JPF0512Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124004JPF0513Encodes pCMV-A driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124005JPF0514Encodes pCMV-B driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124006JPF0515Encodes pCMV-C driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124007JPF0516Encodes pCMV-D driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124008JPF0517Encodes pCMV-E driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124009JPF0518Encodes pCMV-F driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124010JPF0519Encodes pCMV-G driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124011JPF0520Encodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124012JPF0521Encodes crippled pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124013JPF0522Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124014JPF0523Encodes crippled pSFFV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124015ARB287aEncodes pEF1a driving expression of mAzamiGreen with a BgpPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124016ARB287bEncodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124017ARB287cEncodes pEF1a driving expression of mAzamiGreen with a RglPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124018ARB287dEncodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124019ARB287eEncodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124020JPF0419dEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
124021JPF0419vEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
124022JPF0446BxBI Destination vector with H2B::GFP expressed from CAG promoter
124023ARB340Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713
124024ARB341Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFP
124025ARB342Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen
124026ARB343Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mRuby2
124027ARB344Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713
124028ARB345Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFP
124029ARB346Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreen
124030ARB347Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2
124031ARB348Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged iRFP713
124032ARB349Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged tagBFP
124033ARB350Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mAzamiGreen
124034ARB351Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mRuby2
124035JPF0464Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
124036JPF0465Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
124037JPF0466Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
124038JPF0468Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector
124039JPF0454Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector
124040JPF0455Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vector
124041JPF0456Encodes pPGK1 driving expression of mScarlet in a 2nd gen. Lenti Virus destination vector
124042JPF0535aEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector
124048ARB365Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
124049ARB366Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
124050ARB367Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
124054MTK3_028Encodes c120 sg RNA guide cleaved by ribozyme
124055MTK234_047Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
124056MTK234_048Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
124057MTK234_049Encodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system
124058MTK6_016Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system

Antibodies from Article