Low-dose metformin targets the lysosomal AMPK pathway through PEN2.
Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC
Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8. Epub 2022 Feb 23. PubMed Article
Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC
Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8. Epub 2022 Feb 23. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183639 | pBoBi-hPEN2 20A | Lentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1. |
| 183640 | pBoBi-hPEN2 2A | Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face). |
| 183641 | pBoBi-hPEN2 D90A | Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen). |
| 183642 | pBoBi-hAP1 Δ420-440 | To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression |
| 183643 | pcDNA3.3-hAP1 420-440 | To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression |
| 183644 | pcDNA3.3-hAP1 1-419 | To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression |
| 183645 | pBoBi-hAP1-C-LAMP2 TM | Lentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2. |
| 183646 | pBoBi-hLAMP2-C-PEN2 | Lentiviral expression of a lysosome-residential PEN2. |
| 183647 | pBoBi-hTOMM20-C-PEN2 | Lentiviral expression of a mitochondrion-residential PEN2. |
| 183648 | pBoBi-hSEC61B-C-PEN2 | Lentiviral expression of a ER-residential PEN2. |
| 183649 | pBoBi-hGOLGA2-C-PEN2 | Lentiviral expression of a golgi-residential PEN2. |