Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies.
Oliinyk OS, Baloban M, Clark CL, Carey E, Pletnev S, Nimmerjahn A, Verkhusha VV
Nat Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 10.1038/s41592-022-01467-6. PubMed Article
Oliinyk OS, Baloban M, Clark CL, Carey E, Pletnev S, Nimmerjahn A, Verkhusha VV
Nat Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 10.1038/s41592-022-01467-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184663 | pBAD/His-miRFP670nano3 | Monomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmid |
| 184664 | pcDNA-miRFP670nano3 | Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid |
| 184665 | pActin-miRFP670nano3 | Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184666 | pTubulin-miRFP670nano3 | Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184667 | pClathrin-miRFP670nano3 | Clathrin labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184668 | pMyosin-miRFP670nano3 | LC-Myosin labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184669 | pLAMP1-miRFP670nano3 | LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184670 | pH2B-miRFP670nano3 | Histone 2B labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184671 | pB2AR-miRFP670nano3 | Beta-2-adrenergic receptor internally tagged with miRFP670nano3 |
| 184672 | pGas-miRFP670nano3 | G protein alpha-subunit internally tagged with miRFP670nano3 |
| 184673 | pEGFR-miRFP670nano3 | EGF receptor (EGFR) labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184674 | pCytERM-miRFP670nano3 | Signal anchor of rabbit cytochrome p450 and miRFP670nano3 |
| 184675 | pNIR-FbGFP | NIR-Fb to GFP |
| 184676 | pNIR-FbmCherry | NIR-Fb to mCherry |
| 184677 | pNIR-Fbactin | NIR-Fb to actin |
| 184678 | pNIR-FbALFA | NIR-Fb to ALFA-tag |
| 184679 | pNIR-Fb21 | NIR-Fb to spike-Cov-19 RBD |
| 184680 | pNIR-Fb6m | NIR-Fb to spike-Cov-19 RBD |
| 184681 | pNIR-Fb2E7 | NIR-Fb to HIV gp41 |
| 184682 | pNIR-Fb59H10 | NIR-Fb to HIV p24 |
| 184683 | pNIR-Fb4EIG | NIR-Fb to E.coli dihydrofolate reductase |
| 184684 | pNIR-FbGFPY58/C-JIP | NIR-Fb-based JNK inhibitor |
| 184685 | pNIR-FbGFPY58/C-PKI | NIR-Fb-based PKA inhibitor |
| 184686 | pmiRFP670nano3-NbGFP | Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184687 | pNbGFP-miRFP670nano3 | Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3 |
| 184688 | pAAV-hSYN-miRFP670nano3-WPRE-hGHpA | Mammalian expression, miRFP670nano3 |