A multifunctional system for genome editing and large-scale interspecies gene transfer.
Teufel M, Klein CA, Mager M, Sobetzko P
Nat Commun. 2022 Jun 14;13(1):3430. doi: 10.1038/s41467-022-30843-1. PubMed Article
Teufel M, Klein CA, Mager M, Sobetzko P
Nat Commun. 2022 Jun 14;13(1):3430. doi: 10.1038/s41467-022-30843-1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184860 | cr3Ec | cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system |
| 184861 | cr3Vn | cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system |
| 184862 | cr3Dd | cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system |
| 184863 | pSWAP_Entry_T1 | pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system. |
| 184864 | pSWAP_Entry_T2 | pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system. |
| 184865 | pSWAP_Entry_H1_alpha | pSwap plasmid part with H1_alpha homology arms |
| 184866 | pSWAP_Entry_HA | pSwap plasmid part with HA homology arms |
| 184867 | pSWAP_Entry_INS | pSwap plasmid part with insert fragment module |
| 184868 | pSWAP_Entry_HB | pSwap plasmid part with HB homology arms |
| 184869 | pSWAP_Entry_HAIB | For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules. |
| 184870 | pSWAP_Entry_H2_beta_CM | pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette |
| 184871 | pSWAP_Entry_H2_beta_GM | pSwap plasmid part with H2_beta homology arms and gentamycin cassette |
| 184872 | pDrop | Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration. |
| 184873 | pDropVn | Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol. |