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Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi.
Jensen TI, Mikkelsen NS, Gao Z, Fosselteder J, Pabst G, Axelgaard E, Laustsen A, Konig S, Reinisch A, Bak RO
Genome Res. 2021 Nov;31(11):2120-2130. doi: 10.1101/gr.275607.121. Epub 2021 Aug 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
205247dSpCas9-VPRIn vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
205248KRAB-dSpCas9In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.
205251dSaCas9-VPRIn vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

Antibodies from Article