A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins.
May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL, Halfmann CT, Diaz Pena R, Pratt D, Campos AR, Roux KJ
Viruses. 2022 Mar 15;14(3). pii: v14030611. doi: 10.3390/v14030611. PubMed Article
May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL, Halfmann CT, Diaz Pena R, Pratt D, Campos AR, Roux KJ
Viruses. 2022 Mar 15;14(3). pii: v14030611. doi: 10.3390/v14030611. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185986 | N-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 Nucleoprotein fused to BioID2 and HA tag |
| 185987 | E-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 Envelope protein fused to BioID2 and HA tag |
| 185988 | M-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 Membrane protein fused to BioID2 and HA tag |
| 185989 | ORF3a-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 ORF3a fused to BioID2 and HA tag |
| 185990 | ORF6-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 ORF6 fused to BioID2 and HA tag |
| 185991 | ORF7a-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 ORF7a fused to BioID2 and HA tag |
| 185992 | BioID2-1x-ORF7b pBabe-puro (SARS-CoV-2) | Expresses BioID2 fused to SARS-CoV-2 ORF7b and myc tag |
| 185993 | ORF8-1x-TurboID pbabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 ORF8 to TurboID and 3xHA tag |
| 185994 | BioID2-1x-ORF9c pBabe-puro (SARS-CoV-2) | Expresses myc-tagged BioID2 fused to SARS-CoV-2 ORF9c |
| 185995 | ORF10-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 ORF10 fused to BioID2 and HA tag |
| 185996 | NSP2-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP2 fused to BioID2 and HA tag |
| 185997 | BioID2-1x-NSP3 pBabe-puro (SARS-CoV-2) | Expresses myc-tagged BioID2 fused to SARS-CoV-2 NSP3 |
| 185998 | NSP4-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP4 fused to BioID2 and HA tag |
| 185999 | NSP5-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP5 fused to BioID2 and HA tag |
| 186000 | NSP6-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP6 fused to BioID2 and HA tag |
| 186001 | NSP7-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP7 fused to BioID2 and HA tag |
| 186002 | NSP8-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP8 fused to BioID2 and HA tag |
| 186003 | NSP9-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP9 fused to BioID2 and HA tag |
| 186004 | NSP10-1x-BioID2 pbabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP10 fused to BioID2 and HA tag |
| 186005 | NSP11-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP11 fused to BioID2 and HA tag |
| 186006 | BioID2-1x-NSP12 pBabe-puro (SARS-CoV-2) | Expresses myc-tagged BioID2 fused to SARS-CoV-2 NSP12 |
| 186007 | NSP13-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP13 fused to BioID2 and HA tag |
| 186008 | NSP14-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP14 fused to BioID2 and HA tag |
| 186009 | NSP15-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP15 fused to BioID2 and HA tag |
| 186010 | NSP16-1x-BioID2 pBabe-puro (SARS-CoV-2) | Expresses SARS-CoV-2 NSP16 fused to BioID2 and HA tag |
| 186011 | SS-3xHA-TurboID-KDEL pBabe-puro | Expresses TurboID confined to ER by human albumin signal sequence (SS) and KDEL. 3xHA tag. |