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Addgene
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.
Shy BR, Vykunta VS, Ha A, Talbot A, Roth TL, Nguyen DN, Pfeifer WG, Chen YY, Blaeschke F, Shifrut E, Vedova S, Mamedov MR, Chung JJ, Li H, Yu R, Wu D, Wolf J, Martin TG, Castro CE, Ye L, Esensten JH, Eyquem J, Marson A
Nat Biotechnol. 2022 Aug 25:10.1038/s41587-022-01418-8. doi: 10.1038/s41587-022-01418-8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
186054pTR-337 pUC19-tNGFR-P2A-CTLA4 NKnockin of truncated NGFR to target gene
186055pTR-338 pUC19-tNGFR-P2A-LAG3 NKnockin of truncated NGFR to target gene
186056pTR-342 pUC19-tNGFR-P2A-CD28 NKnockin of truncated NGFR to target gene
186057pTR-449 tNGFR-P2A-CCR2Knockin of truncated NGFR to target gene
186058pTR-373 tNGFR-P2A-IL7RKnockin of truncated NGFR to target gene
186059pTR-457 tNGFR-P2A-STAT1Knockin of truncated NGFR to target gene
186060pTR-469 JUNB-tNGFRKnockin of truncated NGFR to target gene
186061pTR-471 IRF4-tNGFRKnockin of truncated NGFR to target gene
186062pTR-472 FOXO1-tNGFRKnockin of truncated NGFR to target gene
186063pTR-473 FOXP1-tNGFRKnockin of truncated NGFR to target gene
186064pTR-345 pUC19-tNGFR-P2A-IL2RB NKnockin of truncated NGFR to target gene
186065pTR-346 pUC19-tNGFR-P2A-IL2RA NKnockin of truncated NGFR to target gene
186066pTR-348 pUC19-tNGFR-P2A-ICOS NKnockin of truncated NGFR to target gene
186067pTR-340 pUC19-tNGFR-P2A-IL2RG NKnockin of truncated NGFR to target gene
186068pTR-374 tNGFR-P2A-B2MKnockin of truncated NGFR to target gene
186069pTR-375 tNGFR-P2A-ITGB7Knockin of truncated NGFR to target gene
186070pTR-459 tNGFR-P2A-STAT6Knockin of truncated NGFR to target gene
186071pTR-377 tNGFR-P2A-CD5Knockin of truncated NGFR to target gene
186072pTR-378 tNGFR-P2A-CCR7Knockin of truncated NGFR to target gene
186073pTR-379 tNGFR-P2A-CD48Knockin of truncated NGFR to target gene
186074pTR-341 pUC19-tNGFR-P2A-PDCD1 NKnockin of truncated NGFR to target gene
186075pTR-460 tNGFR-P2A-STAT4Knockin of truncated NGFR to target gene
186076pTR-458 tNGFR-P2A-STAT2Knockin of truncated NGFR to target gene
186077pTR-339 pUC19-tNGFR-P2A-TIM3 NKnockin of truncated NGFR to target gene
186078pTR-450 tNGFR-P2A-CXCR4Knockin of truncated NGFR to target gene
186080pTR-452 tNGFR-P2A-LckKnockin of truncated NGFR to target gene
186081pTR-454 tNGFR-P2A-CARD11Knockin of truncated NGFR to target gene
186082pTR-455 tNGFR-P2A-WASKnockin of truncated NGFR to target gene
186083pTR-456 tNGFR-P2A-ITGB1Knockin of truncated NGFR to target gene
186085pIG-712pUC19-tNGFR-P2A-ITCHKnockin of truncated NGFR to target gene
186086pIG-747pUC19-tNGFR-P2A-CD7Knockin of truncated NGFR to target gene
186087pIG-735pUC19-tNGFR-P2A-PIK3CDKnockin of truncated NGFR to target gene
186088pIG-723pUC19-tNGFR-P2A-EVER1Knockin of truncated NGFR to target gene
186089pIG-727pUC19-tNGFR-P2A-WASPKnockin of truncated NGFR to target gene
186090pIG-716pUC19-tNGFR-P2A-CD3GKnockin of truncated NGFR to target gene
186091pIG-714pUC19-tNGFR-P2A-SAPKnockin of truncated NGFR to target gene
186092pIG-738pUC19-tNGFR-P2A-Kindlin-3Knockin of truncated NGFR to target gene
186093pIG-731pUC19-tNGFR-P2A-MAGT1Knockin of truncated NGFR to target gene
186094pIG-728pUC19-tNGFR-P2A-WIPKnockin of truncated NGFR to target gene
186095pIG-729pUC19-tNGFR-P2A-MoesinKnockin of truncated NGFR to target gene
186096pIG-702pUC19-tNGFR-P2A-LRBAKnockin of truncated NGFR to target gene
186097pIG-708pUC19-tNGFR-P2A-DOCK8Knockin of truncated NGFR to target gene
186098pIG-719pUC19-tNGFR-P2A-CD3ZKnockin of truncated NGFR to target gene
186099pIG-742pUC19-tNGFR-P2A-Caspase8Knockin of truncated NGFR to target gene
186100pIG-718pUC19-tNGFR-P2A-CD3DKnockin of truncated NGFR to target gene
186101pIG-740pUC19-tNGFR-P2A-BCL10Knockin of truncated NGFR to target gene
186102pIG-704pUC19-tNGFR-P2A-STAT3Knockin of truncated NGFR to target gene
186103pIG-705pUC19-tNGFR-P2A-STAT5bKnockin of truncated NGFR to target gene
186104pIG-736pUC19-tNGFR-P2A-CD40LKnockin of truncated NGFR to target gene
186105pIG-724pUC19-tNGFR-P2A-EVER2Knockin of truncated NGFR to target gene
186106pIG-748pUC19-tNGFR-P2A-AP3B1Knockin of truncated NGFR to target gene
186107pIG-734pUC19-tNGFR-P2A-PIK3R1Knockin of truncated NGFR to target gene
186108pIG-710pUC19-tNGFR-P2A-IL10R1Knockin of truncated NGFR to target gene
186109pIG-713pUC19-tNGFR-P2A-CD45Knockin of truncated NGFR to target gene
186110pIG-730pUC19-tNGFR-P2A-Coronin1AKnockin of truncated NGFR to target gene
186111pIG-717pUC19-tNGFR-P2A-CD3EKnockin of truncated NGFR to target gene
186112pIG-703pUC19-tNGFR-P2A-BACH2Knockin of truncated NGFR to target gene
186113pIG-737pUC19-tNGFR-P2A-CD18Knockin of truncated NGFR to target gene
186114pIG-701pUC19-tNGFR-P2A-FOXP3Knockin of truncated NGFR to target gene
186115pIG-745pUC19-tNGFR-P2A-CD4Knockin of truncated NGFR to target gene
186116pIG-721pUC19-tNGFR-P2A-RhoHKnockin of truncated NGFR to target gene
186117pIG-709pUC19-tNGFR-P2A-IL10Knockin of truncated NGFR to target gene
186118pIG-744pUC19-tNGFR-P2A-CD2Knockin of truncated NGFR to target gene
186119pIG-732pUC19-tNGFR-P2A-STIM1Knockin of truncated NGFR to target gene
186120pIG-720pUC19-tNGFR-P2A-ZAP70Knockin of truncated NGFR to target gene
186121pIG-746pUC19-tNGFR-P2A-CD5Knockin of truncated NGFR to target gene
186122pIG-707pUC19-tNGFR-P2A-TTC37Knockin of truncated NGFR to target gene
186123pIG-648 CTLA4int1-CTLA4-GFP-SV40CTLA4 gene knockin
186124pIG-719 CTLA4-GFP R70WCTLA4 gene knockin with mutation
186125pIG-720 CTLA4-GFP R75WCTLA4 gene knockin with mutation
186126pIG-721 CTLA4-GFP T124PCTLA4 gene knockin with mutation
186127pIG-641 JE-TRAC-BCMA(BB)28z-P2ABCMA-CAR DNA production
186128pTR-177 pUC19-CLTA-N-mCherryknockin of mCherry to CLTA target

Antibodies from Article