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CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression.
Noviello G, Gjaltema RAF, Schulz EG
Nat Commun. 2023 Jun 3;14(1):3225. doi: 10.1038/s41467-023-38909-4. PubMed Article

Plasmids from Article

ID Plasmid Purpose
187945pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-BlasticidindCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187948pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-BlasticidinER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187949pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-BlasticidinSMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187950pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-BlasticidinecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187951pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-BlasticidinFKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187952pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-BlasticidinFKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187953pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-BlasticidinFKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187954pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-BlasticidinFKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187956pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-BlasticidinFKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187957pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-BlasticidinFKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
187959pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36VExpressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.
187960pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
187963pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

Antibodies from Article