Activity-based directed evolution of a membrane editor in mammalian cells.
Tei R, Bagde SR, Fromme JC, Baskin JM
Nat Chem. 2023 May 22. doi: 10.1038/s41557-023-01214-0. PubMed Article
Tei R, Bagde SR, Fromme JC, Baskin JM
Nat Chem. 2023 May 22. doi: 10.1038/s41557-023-01214-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188986 | pCDH-CRY2-mCh-superPLDx10 | A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) |
| 188988 | pCDH-CRY2-mCh-superPLDx30 | A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) |
| 188989 | pCDH-CIBN-CAAX | A lentiviral plasmid encoding plasma membrane-tagged CIBN |
| 188990 | pCDH-p18-CIBN | A lentiviral plasmid encoding lysosome-tagged CIBN |
| 188991 | pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX | A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN |
| 188992 | pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX | A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN |
| 188993 | pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX | A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN |
| 188994 | pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX | A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN |