Compact zinc finger base editors that edit mitochondrial or nuclear DNA in vitro and in vivo.
Willis JCW, Silva-Pinheiro P, Widdup L, Minczuk M, Liu DR
Nat Commun. 2022 Nov 23;13(1):7204. doi: 10.1038/s41467-022-34784-7. PubMed Article
Willis JCW, Silva-Pinheiro P, Widdup L, Minczuk M, Liu DR
Nat Commun. 2022 Nov 23;13(1):7204. doi: 10.1038/s41467-022-34784-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191510 | pB5_R8-3i-ATP8_v8_AGKS | Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left) |
| 191511 | pB7_9-ND51_v8_X1 | Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left) |
| 191512 | pB6_R13-ND51_v8_X1 | Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right) |
| 191513 | pB8_4-3i-ATP8_v8_AGKS | Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right) |
| 191514 | pB6N_RB38-Mt-tk_v8_AGKS | Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAN, Right) |
| 191515 | pB7N_LT51-Mt-tk_v8_AGKS | Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAC, Left) |
| 191516 | pL5_3xG22-DCAF8L2_X1 | Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left) |
| 191517 | pL7_3xG35-EMILIN2_X1 | Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left) |
| 191518 | pL6_3xG34-DCAF8L2_X1 | Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right) |
| 191519 | pL8_3xG34-DCAF8L2_X1 | Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right) |
| 191520 | pL5N_LT32-HBB_X1 | Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAN, Left) |
| 191521 | pL8N_RB610-HBB_X1 | Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAC, Right) |
| 191523 | pAAV-Nd1 | Expression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vector |