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The PROTECTOR strategy employs dCas orthologs to sterically shield off-target sites from CRISPR/Cas activity.
Sapozhnikov DM, Szyf M
Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.1038/s41598-023-29332-2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
191652pLenti-Sa-gRNA-puroLentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.
191653pLenti-eGFP-dSaCas9-blastLentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.
191654pLenti-dSaCas9-KRAB-blastLentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

Antibodies from Article