Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria.
Greenwich JL, Alakavuklar MA, Fuqua C
Microbiol Resour Announc. 2023 Feb 16;12(2):e0111922. doi: 10.1128/mra.01119-22. Epub 2023 Jan 16. PubMed Article

Plasmids from Article

ID Plasmid Purpose
192979pMAT36BHR plasmid (pRA301) expressing mirA-3XFLAG from T7 promoter for high yield protein purification
192980pJLG029BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification
192981pJLG038BHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoter
192982pJLG039BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter

Antibodies from Article