RNA editing of microtubule-associated protein tau circular RNAs promotes their translation and tau tangle formation.
Welden JR, Margvelani G, Arizaca Maquera KA, Gudlavalleti B, Miranda Sardon SC, Campos AR, Robil N, Lee DC, Hernandez AG, Wang WX, Di J, de la Grange P, Nelson PT, Stamm S
Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 10.1093/nar/gkac1129. PubMed Article
Welden JR, Margvelani G, Arizaca Maquera KA, Gudlavalleti B, Miranda Sardon SC, Campos AR, Robil N, Lee DC, Hernandez AG, Wang WX, Di J, de la Grange P, Nelson PT, Stamm S
Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 10.1093/nar/gkac1129. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194161 | pEGFP-C2_3X Flag | Expresses eGFP fluorescent protein with a 3X flag tag |
| 194163 | pcDNA3.1_ZKSCAN1_Tau10-12WT | Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10. |
| 194164 | pcDNA3.1_ZKSCAN1_Tau10-12K317M | Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10. |
| 194165 | pcDNA3.1_ZKSCAN1_Tau10-12V337M | Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau V337M mutation. Expresses the tau circRNA 12-->10 V337M with 3X flag tag in exon 10. |
| 194166 | pcDNA3.1_ZKSCAN1_Tau7-12WT | Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7. |
| 194167 | pcDNA3.1_Tau Authentic Alu 10-12WT | Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 10-12 . Expresses the tau circular RNA 12-->10 WT with 3X flag tag in exon 10. |
| 194168 | pcDNA3.1_Tau Authentic Alu 10-12K317M | Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10. |
| 194169 | pcDNA3.1_Tau Authentic Alu 10-12V337M | Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10. |
| 194170 | pcDNA3.1_Tau Authentic Alu 7-12WT | Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 7,9-12 . Expresses the tau circular RNA 12-->7 WT with 3X flag tag in exon 7. |
| 194171 | pcDNA3.1_Linear Tau 0N4R 3X Flag tag | Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation. |