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Orthogonal CRISPR screens to identify transcriptional and epigenetic regulators of human CD8 T cell function.
McCutcheon SR, Swartz AM, Brown MC, Barrera A, McRoberts Amador C, Siklenka K, Humayun L, Isaacs JM, Reddy TE, Nair S, Antonia S, Gersbach CA
bioRxiv. 2023 May 1:2023.05.01.538906. doi: 10.1101/2023.05.01.538906. Preprint. PubMed Article

Plasmids from Article

ID Plasmid Purpose
194278pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1Expresses gRNA and dSaCas9-KRAB from lentiviral vector
194279pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

Antibodies from Article