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Improved Split TEV GPCR beta-arrestin-2 Recruitment Assays via Systematic Analysis of Signal Peptide and beta-arrestin Binding Motif Variants.
Wu Y, von Hauff IV, Jensen N, Rossner MJ, Wehr MC
Biosensors (Basel). 2022 Dec 29;13(1):48. doi: 10.3390/bios13010048. PubMed Article

Plasmids from Article

ID Plasmid Purpose
194358pcDNA3_DRD1-NTEV-TCS-GV-2xHASplit TEV assays
194359pcDNA3_SP-DRD1-NTEV-TCS-GV-2xHASplit TEV assays
194360pcDNA3_DRD1-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194361pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194362pcDNA3_DRD2-NTEV-TCS-GV-2xHASplit TEV assays
194363pcDNA3_SP-DRD2-NTEV-TCS-GV-2xHASplit TEV assays
194364pcDNA3_DRD2-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194365pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194366pcDNA3_HTR2A-NTEV-TCS-GV-2xHASplit TEV assays
194367pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHASplit TEV assays
194368pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194369pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194370pcDNA3_AVPR2-NTEV-TCS-GV-2xHASplit TEV assays
194371pcDNA3_SP-AVPR2-NTEV-TCS-GV-2xHASplit TEV assays
194372pcDNA3_AVPR2-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194373pcDNA3_SP-AVPR2-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194374pcDNA3_GCGR-NTEV-TCS-GV-2xHASplit TEV assays
194375pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHASplit TEV assays
194376pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194377pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194378pcDNA3_GLP1R-NTEV-TCS-GV-2xHASplit TEV assays
194379pcDNA3_SP-GLP1R-NTEV-TCS-GV-2xHASplit TEV assays
194380pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194381pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHASplit TEV assays
194382pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHASplit TEV assays
194383pGL4_10xUAS-MLPmin-luc2Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter
194384pGL4_CRE-CMVmin-luc2Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
194385pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DESTGateway Destination vector for split TEV assays
194386pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DESTGateway Destination vector for GPCR split TEV assays with V2R tail

Antibodies from Article