Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation.
Powers EN, Chan C, Doron-Mandel E, Llacsahuanga Allcca L, Kim Kim J, Jovanovic M, Brar GA
Elife. 2022 Dec 12;11:e81086. doi: 10.7554/eLife.81086. PubMed Article
Powers EN, Chan C, Doron-Mandel E, Llacsahuanga Allcca L, Kim Kim J, Jovanovic M, Brar GA
Elife. 2022 Dec 12;11:e81086. doi: 10.7554/eLife.81086. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195038 | kanMX6-ins1 | pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference |
| 195039 | kanMX6-ins2 | pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference |
| 195040 | his3MX6-ins3 | pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference |
| 195041 | TRP1MX6-ins4 | pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference |
| 195042 | natMX6-ins5 | pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference |
| 195043 | kanMX6-ins6 | pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference |
| 195044 | natMX-ins7 | pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference |