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R-loop-derived cytoplasmic RNA-DNA hybrids activate an immune response.
Crossley MP, Song C, Bocek MJ, Choi JH, Kousorous J, Sathirachinda A, Lin C, Brickner JR, Bai G, Lans H, Vermeulen W, Abu-Remaileh M, Cimprich KA
Nature. 2023 Jan;613(7942):187-194. doi: 10.1038/s41586-022-05545-9. Epub 2022 Dec 21. PubMed Article

Plasmids from Article

ID Plasmid Purpose
196701pLVX-RNaseHI-NES-EGFPPlasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.
196702pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.
196703pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

Antibodies from Article