Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons.
Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568. PubMed Article
Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197275 | pFsynW SYT1 reporter | Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook). |
| 197276 | pFsynW SYB2 reporter | Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook). |
| 197277 | pFsynW KDEL Hook | Encodes ER-targeted streptavidin-KDEL hook (RUSH system). |
| 197278 | pFsynW SYT1-PGM reporter | Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation. |
| 197279 | pFsynW SYT1deltaC2AB reporter | Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook). |
| 197280 | pEF HaloTag-SYT1 | Encodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTag |
| 197281 | pEF SYT1-HaloTag | Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag |
| 197282 | pEF HaloTag-SYB2 | Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage. |
| 197283 | pFsynW SYP-GFP | Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP. |
| 197284 | pFsynW TeTx-LC | Encodes for viral expression of the light chain of tetanus toxin. |