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Addgene
An extensible vector toolkit and parts library for advanced engineering of plant genomes.
Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R, Gamo ME, Starker CG, Zhang F, Voytas DF
Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
PubMed Article

Some of these materials are included in the Joint Modular Cloning (JMC) Toolkit or the MoClo Plant Parts III: Transformation & Genome Engineering Kit.

Plasmids from Article

ID Plasmid Purpose
197688pMC-1-EXOB-11Phytobrick (MoClo) Level 0 Part
197689pMC-1-TBS-11Phytobrick (MoClo) Level 0 Part
197690pMC-1-Ugi1-11Phytobrick (MoClo) Level 0 Part
197691pMC-1-Ugi3-11Phytobrick (MoClo) Level 0 Part
197692pMC-1-Ugi4-11Phytobrick (MoClo) Level 0 Part
197693pMC-1-Hairpin_Insulator-4Phytobrick (MoClo) Level 0 Part
197694pMC-1-CmYLCV-4Phytobrick (MoClo) Level 0 Part
197695pMC-1-DR5v2-4Phytobrick (MoClo) Level 0 Part
197696pMC-1-OsEf1a-4Phytobrick (MoClo) Level 0 Part
197697pMC-1-Nos-6Phytobrick (MoClo) Level 0 Part
197698pMC-1-35S_Long-6Phytobrick (MoClo) Level 0 Part
197699pMC-1-CmYLCV-6Phytobrick (MoClo) Level 0 Part
197700pMC-1-AtUbi10-6Phytobrick (MoClo) Level 0 Part
197701pMC-1-GmHSP17-5E-6Phytobrick (MoClo) Level 0 Part
197702pMC-1-NtEf1a-6Phytobrick (MoClo) Level 0 Part
197703pMC-1-OsEf1a-6Phytobrick (MoClo) Level 0 Part
197704pMC-1-OsUbi-6Phytobrick (MoClo) Level 0 Part
197705pMC-1-PvUbi1-6Phytobrick (MoClo) Level 0 Part
197706pMC-1-PvUbi2-6Phytobrick (MoClo) Level 0 Part
197707pMC-1-SlEf1a-6Phytobrick (MoClo) Level 0 Part
197708pMC-1-SlRPS5A1-6Phytobrick (MoClo) Level 0 Part
197709pMC-1-SlUbi-6Phytobrick (MoClo) Level 0 Part
197710pMC-1-StUbi-6Phytobrick (MoClo) Level 0 Part
197711pMC-1-ZmAXIG1-6Phytobrick (MoClo) Level 0 Part
197712pMC-1-ZmEf1a-6Phytobrick (MoClo) Level 0 Part
197713pMC-1-ZmPLTP-6Phytobrick (MoClo) Level 0 Part
197714pMC-1-ZmUbi1-6Phytobrick (MoClo) Level 0 Part
197715pMC-6-BeYDV-Rep-RepA-9Phytobrick (MoClo) Level 0 Part
197716pMC-6-WDV-Rep-RepA-9Phytobrick (MoClo) Level 0 Part
197717pMC-1-BeYDV-LS-L-11Phytobrick (MoClo) Level 0 Part
197718pMC-1-BeYDV-LRS-L-11Phytobrick (MoClo) Level 0 Part
197719pMC-1-WDV-LS-L-11Phytobrick (MoClo) Level 0 Part
197720pMC-1-WDV-LRS-L-11Phytobrick (MoClo) Level 0 Part
197721pMC-6-Cre-9Phytobrick (MoClo) Level 0 Part
197722pMC-6-Cre-StLS1-9Phytobrick (MoClo) Level 0 Part
197723pMC-6-Cre-GR-9Phytobrick (MoClo) Level 0 Part
197724pMC-6-FLPe-9Phytobrick (MoClo) Level 0 Part
197725pMC-6-PhiC31-9Phytobrick (MoClo) Level 0 Part
197726pMC-6-R-9Phytobrick (MoClo) Level 0 Part
197727pMC-6-Integrase4-9Phytobrick (MoClo) Level 0 Part
197728pMC-6-Integrase13-9Phytobrick (MoClo) Level 0 Part
197729pMC-6-CinH-9Phytobrick (MoClo) Level 0 Part
197730pMC-6-Bxb1-9Phytobrick (MoClo) Level 0 Part
197731pMC-6-AmCyan-9Phytobrick (MoClo) Level 0 Part
197732pMC-6-Cycle3GFP-9Phytobrick (MoClo) Level 0 Part
197733pMC-6-EGFP-9Phytobrick (MoClo) Level 0 Part
197735pMC-6-DsRed2-9Phytobrick (MoClo) Level 0 Part
197736pMC-6-ELuc-9Phytobrick (MoClo) Level 0 Part
197737pMC-6-FLuc-9Phytobrick (MoClo) Level 0 Part
197738pMC-6-RedFLuc-9Phytobrick (MoClo) Level 0 Part
197739pMC-6-RLuc-9Phytobrick (MoClo) Level 0 Part
197740pMC-6-Ruby-9Phytobrick (MoClo) Level 0 Part
197741pMC-6-GFP-P2A-NptII-9Phytobrick (MoClo) Level 0 Part
197742pMC-6-AmCyan-P2A-NptII-9Phytobrick (MoClo) Level 0 Part
197743pMC-6-HptII-9Phytobrick (MoClo) Level 0 Part
197744pMC-6-NptII-9Phytobrick (MoClo) Level 0 Part
197745pMC-6-AtPLT5-9Phytobrick (MoClo) Level 0 Part
197746pMC-6-IPT-9Phytobrick (MoClo) Level 0 Part
197747pMC-6-TaGRF-GIF-9Phytobrick (MoClo) Level 0 Part
197748pMC-6-ZmBbm-9Phytobrick (MoClo) Level 0 Part
197749pMC-6-ZmWus2-9Phytobrick (MoClo) Level 0 Part
197750pMC-6-ZmBbm-P2A-ZmWus2-9Phytobrick (MoClo) Level 0 Part
197751pMC-6-AsNPGA-9Phytobrick (MoClo) Level 0 Part
197752pMC-6-NnCPH-9Phytobrick (MoClo) Level 0 Part
197753pMC-6-NnH3H-9Phytobrick (MoClo) Level 0 Part
197754pMC-6-NnHISPS-9Phytobrick (MoClo) Level 0 Part
197755pMC-6-NnLuz-9Phytobrick (MoClo) Level 0 Part
197756pMC-6-HpaB-9Phytobrick (MoClo) Level 0 Part
197757pMC-6-HpaC-9Phytobrick (MoClo) Level 0 Part
197758pMC-6-RcTAL-9Phytobrick (MoClo) Level 0 Part
197759pMC-9-35S-11Phytobrick (MoClo) Level 0 Part
197760pMC-9-AtHSP-11Phytobrick (MoClo) Level 0 Part
197761pMC-9-NbAct3-11Phytobrick (MoClo) Level 0 Part
197762pMC-9-NbHSP-11Phytobrick (MoClo) Level 0 Part
197763pMC-9-NtEU-11Phytobrick (MoClo) Level 0 Part
197764pMC-9-NtEf1a-11Phytobrick (MoClo) Level 0 Part
197765pMC-9-OsAct2-11Phytobrick (MoClo) Level 0 Part
197766pMC-9-OsEf1a-11Phytobrick (MoClo) Level 0 Part
197767pMC-9-OsT28-11Phytobrick (MoClo) Level 0 Part
197768pMC-9-OsUbi-11Phytobrick (MoClo) Level 0 Part
197769pMC-9-PinII-11Phytobrick (MoClo) Level 0 Part
197770pMC-9-RBCS-11Phytobrick (MoClo) Level 0 Part
197771pMC-9-SlEf1a-11Phytobrick (MoClo) Level 0 Part
197772pMC-9-SlRPS5A1-11Phytobrick (MoClo) Level 0 Part
197773pMC-9-SlUbi-11Phytobrick (MoClo) Level 0 Part
197774pMC-9-StUbi-11Phytobrick (MoClo) Level 0 Part
197775pMC-9-ZmEf1a-11Phytobrick (MoClo) Level 0 Part
197776pMC-9-ZmIn2-11Phytobrick (MoClo) Level 0 Part
197777pMC-9-ZmUbi-11Phytobrick (MoClo) Level 0 Part
197778pMC-6-AtSpCas9-9Phytobrick (MoClo) Level 0 Part
197779pMC-1-AtU6-YPhytobrick (MoClo) Level 0 Part
197780pMC-1-OsU6-XPhytobrick (MoClo) Level 0 Part
197781pMC-Z-SpCas9-gRNA-repeat-polyT-11Phytobrick (MoClo) Level 0 Part
197782pMIN-0Level 2 destination vector: minimal (non-T-DNA) backbone
197783pMIN-VS1Level 2 destination vector: pMIN-VS1 backbone
197784pMIN-RiLevel 2 destination vector: pMIN-Ri backbone
197785pJMC-1TLevel 1 Module 1T destination vector
197786pJMC-1Level 1 Module 1 destination vector
197787pJMC-2TLevel 1 Module 2T destination vector
197788pJMC-2Level 1 Module 2 destination vector
197789pJMC-3TLevel 1 Module 3T destination vector
197790pJMC-3Level 1 Module 3 destination vector
197791pJMC-4TLevel 1 Module 4T destination vector
197792pJMC-4Level 1 Module 4 destination vector
197793pJMC-5TLevel 1 Module 5T destination vector
197794pJMC-5Level 1 Module 5 destination vector
197795pJMC-6TLevel 1 Module 6T destination vector
197796pJMC-6Level 1 Module 6 destination vector
197797pJMC-7TLevel 1 Module 7T destination vector
197798pJMC-1T-ReverseLevel 1 Module 1T destination vector (reverse orientation MoClo insert)
197799pJMC-1-ReverseLevel 1 Module 1 destination vector (reverse orientation MoClo insert)
197800pJMC-2T-ReverseLevel 1 Module 2T destination vector (reverse orientation MoClo insert)
197801pJMC-2-ReverseLevel 1 Module 2 destination vector (reverse orientation MoClo insert)
197802pJMC-3T-ReverseLevel 1 Module 3T destination vector (reverse orientation MoClo insert)
197803pJMC-3-ReverseLevel 1 Module 3 destination vector (reverse orientation MoClo insert)
197804pJMC-4T-ReverseLevel 1 Module 4T destination vector (reverse orientation MoClo insert)
197805pJMC-4-ReverseLevel 1 Module 4 destination vector (reverse orientation MoClo insert)
197806pJMC-5T-ReverseLevel 1 Module 5T destination vector (reverse orientation MoClo insert)
197807pJMC-5-ReverseLevel 1 Module 5 destination vector (reverse orientation MoClo insert)
197808pJMC-6T-ReverseLevel 1 Module 6T destination vector (reverse orientation MoClo insert)
197809pJMC-6-ReverseLevel 1 Module 6 destination vector (reverse orientation MoClo insert)
197810pJMC-7T-ReverseLevel 1 Module 7T destination vector (reverse orientation MoClo insert)
197811pMC-1-ccdB-6Level 0 J1-J6 destination vector, BbsI entry & CcdB selection
197812pMC-6-ccdB-9Level 0 J6-J9 destination vector, BbsI entry & CcdB selection
197813pMC-9-ccdB-11Level 0 J9-J11 destination vector, BbsI entry & CcdB selection
197814pMC-1-ccdB-4Level 0 J1-J4 destination vector, BbsI entry & CcdB selection
197815pMC-1-ccdB-11Level 0 J1-J11 destination vector, BbsI entry & CcdB selection
197816pJMC-1-BsaILevel 1 module 1 with BsaI expansion point
197817pJMC-2T-BsaILevel 1 module 2T with BsaI expansion point
197818pJMC-2-BsaILevel 1 module 2 with BsaI expansion point
197819pJMC-3T-BsaILevel 1 module 3T with BsaI expansion point
197820pJMC-3-BsaILevel 1 module 3 with BsaI expansion point
197821pJMC-4T-BsaILevel 1 module 4T with BsaI expansion point
197822pJMC-4-BsaILevel 1 module 4 with BsaI expansion point
197823pJMC-5T-BsaILevel 1 module 5T with BsaI expansion point
197824pJMC-5-BsaILevel 1 module 5 with BsaI expansion point
197825pJMC-6T-BsaILevel 1 module 6T with BsaI expansion point
197826pJMC-6-BsaILevel 1 module 6 with BsaI expansion point
197827pJMC-7T-BsaILevel 1 module 7T with BsaI expansion point
197828pJMC-1-Esp3ILevel 1 module 1 with Esp3I expansion point
197829pJMC-2T-Esp3ILevel 1 module 2T with Esp3I expansion point
197830pJMC-2-Esp3ILevel 1 module 2 with Esp3I expansion point
197831pJMC-3T-Esp3ILevel 1 module 3T with Esp3I expansion point
197832pJMC-3-Esp3ILevel 1 module 3 with Esp3I expansion point
197833pJMC-4T-Esp3ILevel 1 module 4T with Esp3I expansion point
197834pJMC-4-Esp3ILevel 1 module 4 with Esp3I expansion point
197835pJMC-5T-Esp3ILevel 1 module 5T with Esp3I expansion point
197836pJMC-5-Esp3ILevel 1 module 5 with Esp3I expansion point
197837pJMC-6T-Esp3ILevel 1 module 6T with Esp3I expansion point
197838pJMC-6-Esp3ILevel 1 module 6 with Esp3I expansion point
197839pJMC-7T-Esp3ILevel 1 module 7T with Esp3I expansion point
197840pJMC-L2-Donor-BsaIDonor vector to restore L2 destination via BsaI
197841pJMC-L2-Donor-Esp3IDonor vector to restore L2 destination via Esp3I
197842pJMC-L1-Donor-Esp3IDonor vector to restore L1 destination via Esp3I
197843pJMC-L2-Reverse-Donor-BsaIDonor vector to restore L2 destination via BsaI (reverse orientation)
197844pJMC-L2-Reverse-Donor-Esp3IDonor vector to restore L2 destination via Esp3I (reverse orientation)
197845pJMC-L1-Reverse-Donor-Esp3IDonor vector to restore L1 destination via Esp3I (reverse orientation)
197846pJMC-1-EmptyLevel 1 module 1 empty
197847pJMC-2T-EmptyLevel 1 module 2T empty
197848pJMC-2-EmptyLevel 1 module 2 empty
197849pJMC-3T-EmptyLevel 1 module 3T empty
197850pJMC-3-EmptyLevel 1 module 3 empty
197851pJMC-4T-EmptyLevel 1 module 4T empty
197852pJMC-4-EmptyLevel 1 module 4 empty
197853pJMC-5T-EmptyLevel 1 module 5T empty
197854pJMC-5-EmptyLevel 1 module 5 empty
197855pJMC-6T-EmptyLevel 1 module 6T empty
197856pJMC-6-EmptyLevel 1 module 6 empty
197857pJMC-7T-EmptyLevel 1 module 7T empty

Antibodies from Article