Adenine Base Editing In Vivo with a Single Adeno-Associated Virus Vector.
Zhang H, Bamidele N, Liu P, Ojelabi O, Gao XD, Rodriguez T, Cheng H, Kelly K, Watts JK, Xie J, Gao G, Wolfe SA, Xue W, Sontheimer EJ
GEN Biotechnol. 2022 Jun 1;1(3):285-299. doi: 10.1089/genbio.2022.0015. Epub 2022 Jun 14. PubMed Article
Zhang H, Bamidele N, Liu P, Ojelabi O, Gao XD, Rodriguez T, Cheng H, Kelly K, Watts JK, Xie J, Gao G, Wolfe SA, Xue W, Sontheimer EJ
GEN Biotechnol. 2022 Jun 1;1(3):285-299. doi: 10.1089/genbio.2022.0015. Epub 2022 Jun 14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199261 | pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2 | Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency. |
| 199262 | pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26 | Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene |
| 199263 | pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah | Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model |
| 199264 | pEJS1831 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Fah | Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model |
| 199265 | pEJS-HZ54 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26 | Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene |