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A highly sensitive NanoLuc-based protease biosensor for detecting apoptosis and SARS-CoV-2 infection.
Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E
Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4. PubMed Article

Plasmids from Article

ID Plasmid Purpose
200119pCAG.CASP3-MycExpresses C-terminal Myc-tagged caspase 3 in mammalian cells
200120pCAG.Myc-SARS2_3CLExpresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells
200121pCAG.Myc-SARS2_3CL(C145A)Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells
200122pQC.Flip-nluc(LgBiT1-8)CP[TEV]Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells
200123pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-FlucRetroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells
200124pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-FlucRetroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells
200125pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-FlucRetroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells
200126pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA]-FlucRetroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells
200127pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-FlucRetroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter and P2A firefly luciferase in mammalian cells
200128pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-FlucRetroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)] protease reporter and P2A firefly luciferase in mammalian cells

Antibodies from Article