The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.
Krastev DB, Li S, Sun Y, Wicks AJ, Hoslett G, Weekes D, Badder LM, Knight EG, Marlow R, Pardo MC, Yu L, Talele TT, Bartek J, Choudhary JS, Pommier Y, Pettitt SJ, Tutt ANJ, Ramadan K, Lord CJ
Nat Cell Biol. 2022 Jan;24(1):62-73. doi: 10.1038/s41556-021-00807-6. Epub 2022 Jan 10. PubMed Article
Krastev DB, Li S, Sun Y, Wicks AJ, Hoslett G, Weekes D, Badder LM, Knight EG, Marlow R, Pardo MC, Yu L, Talele TT, Bartek J, Choudhary JS, Pommier Y, Pettitt SJ, Tutt ANJ, Ramadan K, Lord CJ
Nat Cell Biol. 2022 Jan;24(1):62-73. doi: 10.1038/s41556-021-00807-6. Epub 2022 Jan 10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211552 | PB-PARP1–eGFP | A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette. |
| 211554 | PB-PARP1-del.p119K120S–eGFP | A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette. |
| 211555 | PB-PARP1-Apex2–eGFP | A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette. |
| 211578 | pEGFP-N1-PARP1 | CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection |
| 211579 | pEGFP-N1-PARP1-delM43;F44I | CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection |
| 211580 | pEGFP-N1-PARP1-E988K | CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection |
| 211581 | CHFR-PBZ-eGFP | Expressing CHFR PBZ domain fused to eGFP |