Development of a V5-tag-directed nanobody and its implementation as an intracellular biosensor of GPCR signalling.
Zeghal M, Matte K, Venes A, Patel S, Laroche G, Sarvan S, Joshi M, Rain JC, Couture JF, Giguere PM
J Biol Chem. 2023 Jul 28:105107. doi: 10.1016/j.jbc.2023.105107. PubMed Article
Zeghal M, Matte K, Venes A, Patel S, Laroche G, Sarvan S, Joshi M, Rain JC, Couture JF, Giguere PM
J Biol Chem. 2023 Jul 28:105107. doi: 10.1016/j.jbc.2023.105107. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201473 | NbV5-eGFP | Indirect microscopy/imaging of V5-tagged protein |
| 201474 | NbV5-mCherry | Indirect microscopy/imaging of V5-tagged protein |
| 201475 | NbV5-LgBit | NanoBit detection of V5-tagged protein |
| 201476 | LgBit-NbV5 | NanoBit detection of V5-tagged protein |
| 201477 | NbV5-GFP2 | BRET2 detection of V5-tagged protein |
| 201478 | GFP2-NbV5 | BRET2 detection of V5-tagged protein |
| 201479 | Rluc8-NbV5 | BRET detection of V5-tagged protein |
| 201480 | NbV5-Rluc8 | BRET detection of V5-tagged protein |
| 201481 | NbV5-TEV219 | TANGO detection of V5-tagged protein |
| 201482 | NbV5-HALOTAG | Indirect detection of V5-tagged protein using the HaloTag protein labeling technology. |
| 201483 | NbAlfa-LgBit | NanoBit detection of ALFA-tagged protein |
| 201484 | LgBit-NbALFA | NanoBit detection of ALFA-tagged protein |
| 201485 | NbAlfa-GFP2 | BRET2 detection of ALFA-tagged protein |
| 201486 | GFP2-NbALFA | BRET2 detection of ALFA-tagged protein |
| 201487 | NbALFA-TEV219 | TANGO detection of V5-tagged protein |
| 201488 | NbV5-TEV219S153N | TANGO detection of V5-tagged protein |
| 201489 | NbV5-HiBiT | NanoBit detection of V5-tagged protein |
| 201490 | HiBiT-NbV5 | NanoBit detection of V5-tagged protein |
| 201491 | V5-bArr1 | Expression of the beta-arrestin1 containing a N-terminal V5 tag. |
| 201492 | bArr1-V5 | Expression of the beta-arrestin1 containing a C-terminal V5 tag. |
| 201493 | V5-bArr2 | Expression of the beta-arrestin2 containing a N-terminal V5 tag. |
| 201494 | bArr2-V5 | Expression of the beta-arrestin2 containing a N-terminal V5 tag. |
| 201495 | ALFA-bArr1 | Expression of the beta-arrestin1 containing a N-terminal ALFA tag. |
| 201496 | bArr1-ALFA | Expression of the beta-arrestin1 containing a C-terminal ALFA tag. |
| 201497 | ALFA-bArr2 | Expression of the beta-arrestin2 containing a N-terminal ALFA tag. |
| 201498 | bArr2-ALFA | Expression of the beta-arrestin2 containing a N-terminal ALFA tag. |