Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus.
Chavez M, Rane DA, Chen X, Qi LS
Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x. PubMed Article
Chavez M, Rane DA, Chen X, Qi LS
Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202687 | pSLQ8001 - pCMV-R8.91 (D64V) | R8.91 plasmid with mutated integrase (D64V) |
| 202688 | pSLQ9663 - ACTB GFP-P2A CLIP Donor | CLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms. |
| 202689 | pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor | CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A. |
| 202690 | pSLQ8079 - IL2RG GFP-P2A CLIP Donor | CLIP donor template for integrating GFP into IL2RG locus. |
| 210015 | pSLQ6332 - ACTB CRISPRa CLIP Donor | CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus |