Addgene: Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy.

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Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy.
Lu X, Wang Y, Liu Z, Gou Y, Jaeger D, St-Pierre F
Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
202606	pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX	Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)
202607	pcDNA3.1/Puro-CAG-JEDI-1P	Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)
202608	pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE	Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)
202610	pAAV-EF1a-DIO-tdTomato-WPRE	Double floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)
202611	pAAV-EF1a-DIO-JEDI-1P-WPRE	Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)
202612	pAAV-hSyn-JEDI-1P-Kv-WPRE	Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn
202613	pAAV-hSyn-JEDI-1P-WPRE	Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn
202614	pAAV-CaMKII-JEDI-1P-Kv-WPRE	Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII
202615	pAAV-CaMKII-JEDI-1P-WPRE	Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII
202616	pAAV-GFAP-JEDI-1P-WPRE	Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAP
202617	pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE	Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent
202618	pJFRC7-JEDI-1P	Genetically encoded voltage indicator (GEVI) JEDI-1P under the promoter hsp70 for Drosophila (insect) expression
202619	pHUC-JEDI-1P	Genetically encoded voltage indicator (GEVI) JEDI-1P under the HuC promoter for pan neuronal expression in zebrafish; JEDI-1P sequence codon-optimized for zebrafish expression
202620	pAAV-EF1a-DIO-mCherry-WPRE	Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

Antibodies from Article