Crystal structure of vaccinia virus mRNA capping enzyme provides insights into the mechanism and evolution of the capping apparatus.
Kyrieleis OJ, Chang J, de la Pena M, Shuman S, Cusack S
Structure. 2014 Mar 4;22(3):452-65. doi: 10.1016/j.str.2013.12.014. PubMed Article
Kyrieleis OJ, Chang J, de la Pena M, Shuman S, Cusack S
Structure. 2014 Mar 4;22(3):452-65. doi: 10.1016/j.str.2013.12.014. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203671 | pET-16b-10His-TEV-MCES_VACCA_D1_D12 | The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage |