Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity.
Mahlandt EK, Kreider-Letterman G, Chertkova AO, Garcia-Mata R, Goedhart J
J Cell Sci. 2023 May 15;136(10):jcs260802. doi: 10.1242/jcs.260802. Epub 2023 May 25. PubMed Article
Mahlandt EK, Kreider-Letterman G, Chertkova AO, Garcia-Mata R, Goedhart J
J Cell Sci. 2023 May 15;136(10):jcs260802. doi: 10.1242/jcs.260802. Epub 2023 May 25. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176096 | H2A-mTurquoise-RHOG-QL-deltaCAAX | Constitutively active, nuclear localized RhoG |
| 176099 | dimericTomato-1xwGBD | Visualization of active Cdc42 |
| 176100 | mTurquoise2-3xrGBD | Visualization of active Rho |
| 176109 | HaloTag-2xrGBD | Visualization of active Rho |
| 176110 | H2A-mTurquoise-RhoJQ79L-deltaCaax | Constitutively active, nucelar localized RhoJ |
| 176121 | TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaX | Constitutivley active, mitochondrial localized Cdc42 |
| 176122 | TOMM20-mTurquoise-RhoG-QL-deltaCaaX | Constitutively active, mitochondiral localized RhoG |
| 176123 | TOMM20-mTurquoise-RhoA-G14V-deltaCaaX | Constitutively active, mitochondrial localized RhoA |
| 176124 | TOMM20-mTurquoise-Rac1-G12V-deltaCaaX | Constitutively active, mitochondrial localized Rac1 |
| 176136 | pEntr1a-wGBD-mTurquoise2-wGBD | Visualization of active Cdc42 |
| 176137 | pEntra1a-mTurquoise2-3xrGBD | Visualization of active Rho |
| 191450 | CMVdel-dimericTomato-WASp(CRIB) | Visualizing endogenous & active Cdc42 |
| 191451 | N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB) | Visualizing endogenous & active Cdc42 |
| 191452 | WASp(CRIB)-HaloTag-WASp(CRIB) | Visualizing endogenous & active Cdc42 |