Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Programmable protein expression using a genetically encoded m(6)A sensor.
Marayati BF, Thompson MG, Holley CL, Horner SM, Meyer KD
Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01978-3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
204765pGEMS-IIGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)
204766pGEMS-II-PESTGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
204767pGEMS-II-dCas13-APO1-YTHGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)
204768pU6-PspCas13b-GEMSgRNA2pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

Antibodies from Article