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Addgene
Annette Dolphin Lab: Dolphin Lab Plasmids 2023
Unpublished

Plasmids from Article

ID Plasmid Purpose
206055GFP Cav2.2 HA L702 P pCAGGSexpression of rabbit Cav2.2 with N-terminal GFP tag and exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional
206056GFP Cav2.2 HA L702P pcDNA3expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional
206073Cav2.2 pHfluorin pCAGGSexpression of rabbit Cav2.2 calcium channel with an exofacial pHfluorin tag in domain II
206090mEos Cav2.2 W391A pRK5expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2
206091mEos Cav2.2 pRK5expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag
206092mEos Cav2.2 pMT2expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag
206102cacnb1b pCAGGSexpression of rat beta1b calcium channel auxillary subunit
206103cacna2d1 pcDNA3expression of rat alpha2delta-1 calcium channel auxillary subunit
206104cacna2d1 pCAGGSexpression of rat alpha2delta-1 calcium channel auxillary subunit
206105cacnb1b pcDNA3expression of rat beta1b calcium channel auxillary subunit
206109cacna2d1 R241A D491A HA pcDNA3expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding
206113GFP Cav2.2 HA D665A pcDNA3expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional
206117cacna2d3 BBS pMT2expression of mouse calcium channel alpha2delta-3 with a single Bungarotoxin binding site motif inserted after aa595

Antibodies from Article