MRE11 liberates cGAS from nucleosome sequestration during tumorigenesis.
Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis JE, Gupta GP
Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6. PubMed Article
Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis JE, Gupta GP
Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208382 | Lenti-sgRNA-Cre-GpNLuc | Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2. |
| 208383 | Lenti-sgRNA-Cre-GpNluc-sgControl | This sgControl, located in the TP53BP1 intron, serves as a control sgRNA for the others. The vector was cloned from Lenti-sgRNA-Cre-GpNLuc. |
| 208384 | Lenti-sgRNA-Cre-GpNLuc-sgmMRE11 | Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene. |
| 208385 | Lenti-sgRNA-Cre-GpNLuc-sgmcGAS | Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene. |
| 208386 | Lenti-sgRNA-Cre-GpNLuc-sgmSTING | Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene. |
| 208387 | Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#1 | Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#1 gene. |
| 208388 | Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2 | Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#2 gene. |
| 208389 | Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3 | Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene. |
| 208391 | Lenti-sgRNA-Cre-GpNLuc-sgmMLKL | Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MLKL gene. |
| 208392 | Lenti-sgRNA-Cre-GpNLuc-sgmATM | Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ATM gene. |
| 208393 | pBABE-puro-Halo-hMRE11(wt) | To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection. |
| 208394 | pBABE-puro-Halo-hMRE11(H129N) | To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection. |
| 208395 | pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ) | To generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection. |
| 208396 | PB-TA-ERP2-hcGAS(wt) | To generate stable cell line expressing human cGAS(wt) using PiggyBac transposon system |
| 208397 | PB-TA-ERP2-hcGAS(R255A) | To generate stable cell line expressing human cGAS(R255A) using PiggyBac transposon system |