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Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells.
Makri Pistikou AM, Cremers GAO, Nathalia BL, Meuleman TJ, Bogels BWA, Eijkens BV, de Dreu A, Bezembinder MTH, Stassen OMJA, Bouten CCV, Merkx M, Jerala R, de Greef TFA
Nat Commun. 2023 Nov 2;14(1):7001. doi: 10.1038/s41467-023-42810-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
209109pLeo-A-GEMS(JAK-STAT)Transient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)
209110pLeo-A-a2-GEMS(JAK-STAT)Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)
209111pLeo-A-a3-GEMS(JAK-STAT)Transient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)
209112pLeo-B-a2-GEMS(JAK-STAT)Transient mammalian expression of the CC functionalized GEMS receptor B-a2-GEMS (JAK-STAT)
209113pLeo-G-a2-GEMS(JAK-STAT)Transient mammalian expression of the CC functionalized GEMS receptor G-a2-GEMS (JAK-STAT)
209114pLeo-A-a2-GEMS(PLCG)Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (PLCG)
209115pET28a(+)-SUMO-A'Bacterial expression of CC-GEMS ligand SUMO-A'
209116pET28a(+)-SUMO-A'-l1-A'Bacterial expression of CC-GEMS ligand SUMO-A'-l1-A'
209117pET28a(+)-SUMO-A'-l2-A'Bacterial expression of CC-GEMS ligand SUMO-A'-l2-A'
209118pET28a(+)-SUMO-A'-l3-A'Bacterial expression of CC-GEMS ligand SUMO-A'-l3-A'
209119pET28a(+)-SUMO-B'-l2-B'Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'
209120pET28a(+)-SUMO-G'-l2-G'Bacterial expression of CC-GEMS ligand SUMO-G'-l2-G'
209121pET28a(+)-SUMO-A'-l2-B'Bacterial expression of CC-GEMS ligand SUMO-A'-l2-B'
209122pET28a(+)-SUMO-A'-l2-G'Bacterial expression of CC-GEMS ligand SUMO-A'-l2-G'
209123pET28a(+)-SUMO-A'-l4-GBacterial expression of CC-GEMS ligand SUMO-A'-l4-G
209124pET28a(+)-SUMO-A'-l4-G'Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G'
209125pHR-SUMO-A'-l2-A'-IRES-EmGFPLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP
209126pHR-SUMO-A'-l4-G-IRES-EmGFPLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP
209127pHR-SUMO-A'-l4-G'-IRES-EmGFPLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP
209128pLS13-IL-10Transient mammalian expression of IL-10

Antibodies from Article