A noncanonical function of SKP1 regulates the switch between autophagy and unconventional secretion.
Li J, Krause GJ, Gui Q, Kaushik S, Rona G, Zhang Q, Liang FX, Dhabaria A, Anerillas C, Martindale JL, Vasilyev N, Askenazi M, Ueberheide B, Nudler E, Gorospe M, Cuervo AM, Pagano M
Sci Adv. 2023 Oct 13;9(41):eadh1134. doi: 10.1126/sciadv.adh1134. Epub 2023 Oct 13. PubMed Article
Li J, Krause GJ, Gui Q, Kaushik S, Rona G, Zhang Q, Liang FX, Dhabaria A, Anerillas C, Martindale JL, Vasilyev N, Askenazi M, Ueberheide B, Nudler E, Gorospe M, Cuervo AM, Pagano M
Sci Adv. 2023 Oct 13;9(41):eadh1134. doi: 10.1126/sciadv.adh1134. Epub 2023 Oct 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 212043 | Flag-SKP1-WT | Expresses human SKP1 in mammalian cells |
| 213423 | Flag-SKP1-K142R | Expresses human SKP1 K142R mutant in mammalian cells |
| 213424 | Flag-SKP1-K163R | Expresses human SKP1 K163R mutant in mammalian cells |
| 213425 | Flag-SKP1-RR (K142R/K163R) | Expresses human SKP1 K142R/K163R mutant in mammalian cells |
| 213482 | Flag-SKP1-T131A | Expresses human SKP1 T131A mutant in mammalian cells |
| 213600 | Flag-SKP1-T131D | Expresses human SKP1 T131D mutant in mammalian cells |
| 213601 | Flag-SKP1-T131E | Expresses human SKP1 T131E mutant in mammalian cells |
| 213642 | Flag-SKP1-97-101-5A | Expresses human SKP1-97-101-5A mutant in mammalian cells |
| 213643 | Flag-SKP1-113-117-5A | Expresses human SKP1-113-117-5A mutant in mammalian cells |
| 213644 | Flag-SKP1-120-124-5A | Expresses human SKP1-120-124-5A mutant in mammalian cells |
| 213645 | Flag-SKP1-138-142-5A | Expresses human SKP1-138-142-5A mutant in mammalian cells |
| 213687 | Flag-SKP1-F139A/I141A | Expresses human SKP1-F139A/I141A mutant in mammalian cells |
| 213690 | pET-24d-Flag-SKP1 | Expresses human SKP1 in bacteria cells |
| 213734 | pBabe-Flag-SKP1-T131A | Expresses human SKP1 T131A mutant in mammalian cells |