Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis.
Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
Mol Cell. 2023 Oct 8:S1097-2765(23)00753-0. doi: 10.1016/j.molcel.2023.09.026. PubMed Article
Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
Mol Cell. 2023 Oct 8:S1097-2765(23)00753-0. doi: 10.1016/j.molcel.2023.09.026. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211459 | pcDNA3-ApaLID211A-HA | Express mitochondrial localized ApaLI with D211A mutation |
| 211460 | pcDNA3-ApaLIE225A, K227A-HA | Express mitochondrial localized ApaLI with E225A and K227A mutations |
| 211461 | pcDNA3-ApaLI*-HA | Express mitochondrial localized ApaLI with D211A, E225A and K227A mutations |
| 211462 | TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro | Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI |
| 211463 | TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro | Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A) |
| 211465 | lenti-CMV-TFAM-BioID2-IRES-Puro | Lentivirus backbone expressing human TFAM fused with BioID2 |
| 211466 | pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10 | Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal |
| 211467 | pHAGE2-EF1a-GFP1-10-IRES-Puro | Lentivirus backbone expressing GFP1-10 |
| 211468 | pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro | Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal |
| 211469 | pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro | Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal |