Transposon-encoded nucleases use guide RNAs to promote their selfish spread.
Meers C, Le HC, Pesari SR, Hoffmann FT, Walker MWG, Gezelle J, Tang S, Sternberg SH
Nature. 2023 Sep 27. doi: 10.1038/s41586-023-06597-1. PubMed Article
Meers C, Le HC, Pesari SR, Hoffmann FT, Walker MWG, Gezelle J, Tang S, Sternberg SH
Nature. 2023 Sep 27. doi: 10.1038/s41586-023-06597-1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210869 | pSL4529 (pTnpA) | Expresses GstTnpA from pLac promoter |
| 210870 | pSL4624 (pEffector_TnpB1,ωRNA) | Expresses GstTnpB1 and native ωRNA from constitutive promoters |
| 210871 | pSL4369 (pEffector_TnpB2,ωRNA) | Expresses GstTnpB2 and native ωRNA from constitutive promoters |
| 210872 | pSL4672 (pEffector_TnpB3,ωRNA) | Expresses GstTnpB3 and native ωRNA from constitutive promoters |
| 210873 | pSL4673 (pEffector_TnpB4,ωRNA) | Expresses GstTnpB4 and native ωRNA from constitutive promoters |
| 210874 | pSL4164 (pEffector_IscB,ωRNA) | Expresses GstIscB and native ωRNA from constitutive promoters |
| 210875 | pSL5200 (pDonor_ISGst3) | Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458 |