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Transcriptional linkage analysis with in vivo AAV-Perturb-seq.
Santinha AJ, Klingler E, Kuhn M, Farouni R, Lagler S, Kalamakis G, Lischetti U, Jabaudon D, Platt RJ
Nature. 2023 Oct;622(7982):367-375. doi: 10.1038/s41586-023-06570-y. Epub 2023 Sep 20. PubMed Article

Plasmids from Article

ID Plasmid Purpose
211502pAS088AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.
211503pAS132AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
211504pAS133AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
211505pAS134AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
211506pAS006AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

Antibodies from Article