Kang Zhou Lab:
Harnessing xylitol utilization in Escherichia coli for antibiotic-free plasmid and recombinant protein production.
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 212138 | INT_Cargo_PgyrA_gfp | Cargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C. |
| 212139 | INT_Cargo_T7_gutB | Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C. |
| 212142 | INT_Cargo_PgyrA_katG_dadA6 | Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C. |
| 212143 | INT_GFPsp_CamRcargo | Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C. |
| 212144 | INT_GFPsp | Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C. |
| 212145 | INT_SS9sp | Effector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C. |
| 212146 | PgyrA_gfp | Plasmid expressing green fluorescent protein under constitutive promoter PgyrA control. |
| 212148 | pGU06 | Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control. |
| 212149 | pGU08 | Plasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid. |
| 212150 | pCT03 | Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C. |
| 212152 | pCT02 | Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control. |
| 212153 | PHB1 | For PHB production in E. coli (PT7-pMB1). |
| 212155 | P48 | Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control. |