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Molecular basis and cellular functions of vinculin-actin directional catch bonding.
Chirasani VR, Khan MAI, Malavade JN, Dokholyan NV, Hoffman BD, Campbell SL
Nat Commun. 2023 Dec 14;14(1):8300. doi: 10.1038/s41467-023-43779-x. PubMed Article

Plasmids from Article

ID Plasmid Purpose
213411pRRL-VcnTS-E1015A-E1021AVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.
213412pcDNA3.1-VcnCS-E1015AVinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
213413pcDNA3.1-VcnCS-E1021AVinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).
213414pcDNA3.1-VcnCS-E1015A-E1021AVinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).
213415pcDNA3.1-VcnTS-E1015AVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
213416pcDNA3.1-VcnTS-E1021AVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).
213417pcDNA3.1-VcnTS-E1015A-E1021AVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

Antibodies from Article