A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit.
Waters CS, Angenent SB, Altschuler SJ, Wu LF
Cell Rep. 2023 Oct 31;42(10):113260. doi: 10.1016/j.celrep.2023.113260. Epub 2023 Oct 17. PubMed Article
Waters CS, Angenent SB, Altschuler SJ, Wu LF
Cell Rep. 2023 Oct 31;42(10):113260. doi: 10.1016/j.celrep.2023.113260. Epub 2023 Oct 17. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213544 | pCMV-mKO2-Parkin | Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact |
| 213545 | pCMV-Parkin(C431N)-A92mKO2 | Transient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2 |
| 213546 | pCMV-Parkin(E16A) | Transient mammalian expresssion of E16A Parkin |
| 213547 | pCMV-Parkin | Transient mammalian expresssion of untagged Parkin |
| 213548 | pCMV-Parkin-A92mKO2 | Transient mammalian expresssion of Parkin, internally tagged with mKO2 |
| 213549 | pLv-CMV(trunc)-Parkin-A92mKO2 | Lentiviral vector for stable low expression of Parkin, internally tagged with mKO2 |
| 213554 | pLv-EF1a-Parkin(K161N)-A92mKO2 | Lentiviral vector for stable expression of K161N Parkin, internally tagged with mKO2 |
| 213555 | pLv-EF1a-Parkin(N273K)-A92mKO2 | Lentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2 |
| 213556 | pLv-EF1a-Parkin(R104A)-A92mKO2 | Lentiviral vector for stable expression of R104A Parkin, internally tagged with mKO2 |
| 213557 | pLv-EF1a-Parkin(S65A)-A92mKO2 | Lentiviral vector for stable expression of S65A Parkin, internally tagged with mKO2 |
| 213558 | pLv-EF1a-Parkin(W403A)-A92mKO2 | Lentiviral vector for stable expression of W403A Parkin, internally tagged with mKO2 |
| 213559 | pLv-EF1a-Parkin-A92mKO2 | Lentiviral vector for stable expression of Parkin, internally tagged with mKO2 |