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Synaptotagmin-7 outperforms synaptotagmin-1 to promote the formation of large, stable fusion pores via robust membrane penetration.
Courtney KC, Mandal T, Mehta N, Wu L, Li Y, Das D, Cui Q, Chapman ER
Nat Commun. 2023 Nov 27;14(1):7761. doi: 10.1038/s41467-023-42497-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
213619pET SUMO-syt1 (96-421)Bacterial expression of SUMO-tagged rat syt1 (96-421)
213620pET SUMO-syt1 (96-421) I367CBacterial expression of SUMO-tagged rat syt1 (96-421) I367C
213621pET SUMO-syt7 (136-403) Bacterial expression of SUMO-tagged mouse syt7 (136-403)
213622pET SUMO-syt7 (136-403) L361C Bacterial expression of SUMO-tagged mouse syt7 (136-403) L361C
213623pET SUMO-syt1 5WBacterial expression of SUMO-tagged rat syt1 5W
213624pET SUMO-syt1 s7+Bacterial expression of SUMO-tagged rat syt1 s7+
213625pET SUMO-syt7 C2AB - 4ABacterial expression of SUMO-tagged mouse syt7 C2AB - 4A
213626pET SUMO-syt7 C2A-2A 2BBacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B
213627pET SUMO-syt7 C2AB-2ABacterial expression of SUMO-tagged mouse syt7 C2AB-2A
213628pET SUMO-FL-syt1Bacterial expression of SUMO-tagged rat FL-syt1
213629pET SUMO-FL-syt1 5WBacterial expression of SUMO-tagged rat FL-syt1 5W
213630pET SUMO-FL-syt7Bacterial expression of SUMO-tagged mouse FL-syt7
213631pET SUMO-Doc2β C2AB (G361C)Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)
213633pET SUMO-cPLA2 C2 domain (Y96C)Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)

Antibodies from Article