Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization.
Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT
Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5. PubMed Article
Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT
Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215401 | AfNitB_pET29b | Expression construct for AfNitB gene, codon optimized for expression in E. coli |
| 215402 | AoC_pET29b | Expression construct for AoC gene, codon optimized for expression in E. coli |
| 215403 | BcC _pET29b | Expression construct for BcC gene, codon optimized for expression in E. coli |
| 215404 | Cut190*_pET21b | Expression construct for Cut190* gene, codon optimized for expression in E. coli |
| 215405 | FsC_pET29b | Expression construct for FsC gene, codon optimized for expression in E. coli |
| 215406 | GatA_pET29b | Expression construct for GatA gene, codon optimized for expression in E. coli |
| 215407 | HotPETase_pET21b | Expression construct for HotPETase gene, codon optimized for expression in E. coli |
| 215408 | LCC-ICCG_pET21b | Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli |
| 215409 | M-NylC_pET28a | Expression construct for M-NylC gene, codon optimized for expression in E. coli |
| 215410 | MGS0156_pET29b | Expression construct for MGS0156 gene, codon optimized for expression in E. coli |
| 215411 | MsmegA _pET29b | Expression construct for MsmegA gene, codon optimized for expression in E. coli |
| 215412 | Nc-NylC_pET28a | Expression construct for Nc-NylC gene, codon optimized for expression in E. coli |
| 215413 | NfPolyA_pET21b | Expression construct for NfPolyA gene, codon optimized for expression in E. coli |
| 215414 | NylB_pET29b | Expression construct for NylB gene, codon optimized for expression in E. coli |
| 215415 | NylB-SCY_pET29b | Expression construct for NylB-SCY gene, codon optimized for expression in E. coli |
| 215416 | NylB'_pET21b | Expression construct for NylB' gene, codon optimized for expression in E. coli |
| 215417 | NylB'-SCY_pET21b | Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli |
| 215418 | NylCA_pET28a | Expression construct for NylCA gene, codon optimized for expression in E. coli |
| 215419 | NylCA-TS_pET28a | Expression construct for NylCA-TS gene, codon optimized for expression in E. coli |
| 215420 | NylCp2_pET28a | Expression construct for NylCp2 gene, codon optimized for expression in E. coli |
| 215421 | NylCp2-TS_pET28a | Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli |
| 215422 | NylCK_pET28a | Expression construct for NylCK gene, codon optimized for expression in E. coli |
| 215423 | NylCK-TS_pET28a | Expression construct for NylCK-TS gene, codon optimized for expression in E. coli |
| 215424 | OoH_pET29b | Expression construct for OoH gene, codon optimized for expression in E. coli |
| 215425 | Ph-NylC_pET28a | Expression construct for Ph-NylC gene, codon optimized for expression in E. coli |
| 215426 | ReU_pET29b | Expression construct for ReU gene, codon optimized for expression in E. coli |
| 215427 | RoL10X_pET29b | Expression construct for RoL10X gene, codon optimized for expression in E. coli |
| 215429 | ThcCut1_pET29b | Expression construct for ThcCut1 gene, codon optimized for expression in E. coli |
| 215430 | ThcCut2 _pET29b | Expression construct for ThcCut2 gene, codon optimized for expression in E. coli |
| 215431 | Tt-NylC_pET28a | Expression construct for Tt-NylC gene, codon optimized for expression in E. coli |
| 215432 | TtA_pET29b | Expression construct for TtA gene, codon optimized for expression in E. coli |
| 215433 | UMG-SP-1_pET29b | Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli |
| 215434 | UMG-SP-2_pET29b | Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli |
| 215435 | UMG-SP-3_pET29b | Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli |