Kai Wang Lab:
Kai Wang Lab Plasmids January 2024
Zhao Y, Wang K
unpublished
Zhao Y, Wang K
unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215543 | SOX2-3'gRNA | gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in |
| 215544 | SOX2-P2A-NLS-mClover3-IRES-Puro | Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus |
| 215545 | B2M-SDMutation-EPG | Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out |
| 215546 | B2M-SDMutation-EhT | Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out |
| 215547 | B2M-SDMutation-gRNA | gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus |
| 215548 | P2A-NLS-tdtomato-EPG backbone | An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP |
| 215549 | P2A-mCherry-EhT backbone | An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato |
| 215550 | PBAN-iETV2-IRES-mCherry | Dox-inducible expression of ETV2 for endothelial cell differentiation |