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Addgene
Modular vector assembly enables rapid assessment of emerging CRISPR technologies.
McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller NW, Steger RJ, Escude Velasco B, Bosch JA, Zirin JD, Viswanatha R, Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench JG
Cell Genom. 2024 Mar 13;4(3):100519. doi: 10.1016/j.xgen.2024.100519. PubMed Article

Plasmids from Article

ID Plasmid Purpose
215927AA016Fragmid fragment: (guide cassette) guide expression
215928AA017Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA
215929AA021Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215930AA022Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA
215931AA023Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215932AA054Fragmid fragment: (guide cassette) guide expression; reverse orientation
215933AA074Fragmid fragment: (guide cassette) Tet-regulatable guide expression; positive control cell surface
215934AA083Fragmid fragment: (guide cassette) reverse orientation; guide expression; positive control cell surface
215935AA102Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs
215936AA107Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs
215937AA145Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)
215938AA151Fragmid fragment: (guide cassette) for dual expression sgRNA
215939AA179Fragmid fragment: (guide cassette) short sequence with no known function
215940AA232Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
215941AA233Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
215942AA238Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215943AA242Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215944AA243Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215945AA244Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215946AA246Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215947AA285Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215948AA286Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215949AA290Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215950AA310Fragmid fragment: (guide cassette) guide expression; positive control lethal
215951AA315Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal
215952AA320Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface
215953AA324Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface
215954AA331Fragmid fragment: (guide cassette) guide expression
215955AA363Fragmid fragment: (guide cassette) guide expression
215956AA364Fragmid fragment: (guide cassette) epegRNA expression
215957AA390Fragmid fragment: (guide cassette) guide expression; positive control reporter
215958AA400Fragmid fragment: (guide cassette) guide expression
215959AA401Fragmid fragment: (guide cassette) guide expression
215960AA403Fragmid fragment: (guide cassette) guide expression
215961AA427Fragmid fragment: (guide cassette) enables iBar UMIs
215962AA435Fragmid fragment: (guide cassette) guide expression; positive control cell surface
215963AA041Fragmid fragment: (Pol 2 promoter) human core (short) EF1a promoter
215964AA043Fragmid fragment: (Pol 2 promoter) human Phosphoglycerate Kinase promoter
215965AA065Fragmid fragment: (Pol 2 promoter) human EF1a promoter
215966AA066Fragmid fragment: (Pol 2 promoter) human UbiquitinC promoter
215967AA265Fragmid fragment: (Pol 2 promoter) spleen focus-forming virus promoter
215968AA294Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter
215969AA365Fragmid fragment: (Pol 2 promoter) Gal4-induced expression
215970AA366Fragmid fragment: (Pol 2 promoter) Gal4-induced expression
215971AA367Fragmid fragment: (Pol 2 promoter) Copper-induced expression
215972AA368Fragmid fragment: (Pol 2 promoter) ubiquitous expression
215973AA011Fragmid fragment: (N' terminus) for C>T base editing
215974AA012Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal
215975AA013Fragmid fragment: (N' terminus) for A>G base editing
215976AA018Fragmid fragment: (N' terminus) 4xVP16 transcritional activators
215977AA081Fragmid fragment: (N' terminus) HIV Nuclear Export Sequence
215978AA088Fragmid fragment: (N' terminus) ZIM3 KRAB
215979AA091Fragmid fragment: (N' terminus) ZNF10 KRAB
215980AA122Fragmid fragment: (N' terminus) 4xVP16 transcritional activators
215981AA124Fragmid fragment: (N' terminus) catalytic core of human acetyltransferase p300
215982AA125Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb
215983AA177Fragmid fragment: (N' terminus) Start codon only
215984AA195Fragmid fragment: (N' terminus) ALFA tag for recruiting via NbALFA
215985AA198Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb
215986AA295Fragmid fragment: (N' terminus) SV40 Nuclear Localization Signal
215987AA369Fragmid fragment: (N' terminus) attenuate expression
215988AA370Fragmid fragment: (N' terminus) attenuate expression
215989AA371Fragmid fragment: (N' terminus) attenuate expression
215990AA413Fragmid fragment: (N' terminus) conditional degradation
215991AA414Fragmid fragment: (N' terminus) conditional degradation
215992AA416Fragmid fragment: (N' terminus) luminescent reporter
215993AA067Fragmid fragment: (Selection) hygromycin resistance
215994AA068Fragmid fragment: (Selection) puromycin resistance
215995AA069Fragmid fragment: (Selection) blasticidin resistance
215996AA070Fragmid fragment: (Selection) violet-excited GFP
215997AA082Fragmid fragment: (Selection) fluorescent reporter
215998AA104Fragmid fragment: (Selection) surface marker
215999AA277Fragmid fragment: (Selection) neomycin resistance
216000AA278Fragmid fragment: (Selection) fluorescent reporter
216001AA279Fragmid fragment: (Selection) fluorescent reporter
216002AA007Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM
216003AA008Fragmid fragment: (Cas protein) preferred over wildtype Cas12a
216004AA020Fragmid fragment: (Cas protein) binds PP7 stem loops
216005AA025Fragmid fragment: (Cas protein) NG PAM
216006AA026Fragmid fragment: (Cas protein) NG PAM
216007AA027Fragmid fragment: (Cas protein) nearly PAM-less
216008AA028Fragmid fragment: (Cas protein) nickase Cas for base editing
216009AA029Fragmid fragment: (Cas protein) nickase Cas for base editing
216010AA030Fragmid fragment: (Cas protein) nickase Cas for base editing
216011AA031Fragmid fragment: (Cas protein) nickase Cas for base editing
216012AA032Fragmid fragment: (Cas protein) deactivated Cas
216013AA033Fragmid fragment: (Cas protein) deactivated Cas
216014AA034Fragmid fragment: (Cas protein) deactivated Cas
216015AA035Fragmid fragment: (Cas protein) deactivated Cas
216016AA048Fragmid fragment: (Cas protein) deactivated Cas
216017AA060Fragmid fragment: (Cas protein) nickase Cas for prime editing
216018AA061Fragmid fragment: (Cas protein) nickase Cas for prime editing
216019AA062Fragmid fragment: (Cas protein) nickase Cas for prime editing
216020AA063Fragmid fragment: (Cas protein) nickase Cas for prime editing
216021AA076Fragmid fragment: (Cas protein) Cas13d
216022AA156Fragmid fragment: (Cas protein) Nanobody binds ALFA tag
216023AA180Fragmid fragment: (Cas protein) NGRR(T) PAM
216024AA181Fragmid fragment: (Cas protein) unmodified AsCas12a; not preferred
216025AA183Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency
216026AA201Fragmid fragment: (Cas protein) firefly luciferase
216027AA226Fragmid fragment: (Cas protein) Cas7-11
216028AA296Fragmid fragment: (Cas protein) NNNNCC PAM
216029AA299Fragmid fragment: (Cas protein) NNGG PAM
216030AA301Fragmid fragment: (Cas protein) NNGG PAM
216031AA345Fragmid fragment: (Cas protein) Cas13d
216032AA349Fragmid fragment: (Cas protein) Renilla luciferase
216033AA391Fragmid fragment: (Cas protein) reverse TetR; binds to TRE in presence of doxycycline
216035AA436Fragmid fragment: (Cas protein) binds MS2 stem loops
216036AA440Fragmid fragment: (Cas protein) binds to TRE in absence of doxycycline
216037AA009Fragmid fragment: (C' terminus) Nucleoplasmin NLS
216038AA010Fragmid fragment: (C' terminus) for C>T base editing
216040AA058Fragmid fragment: (C' terminus) for prime editing
216041AA092Fragmid fragment: (C' terminus) ZIM3 KRAB
216042AA093Fragmid fragment: (C' terminus) ZIM3 KRAB
216043AA095Fragmid fragment: (C' terminus) ZNF10 KRAB
216044AA134Fragmid fragment: (C' terminus) SV40NLS; Linker; MeCP2
216045AA141Fragmid fragment: (C' terminus) SV40NLS; Linker; p300
216046AA178Fragmid fragment: (C' terminus) Stop only
216047AA184Fragmid fragment: (C' terminus) for prime editing; using PEmax
216048AA185Fragmid fragment: (C' terminus) for prime editing; using PEmax; for PE4 & 5
216049AA197Fragmid fragment: (C' terminus) ALFA; ALFA; ALFA; ALFA; ALFA; NpNLS; 3xHA
216050AA199Fragmid fragment: (C' terminus) p65; NpNLS
216051AA200Fragmid fragment: (C' terminus) p65; HSF1; NpNLS
216052AA302Fragmid fragment: (C' terminus) Nucleoplasmin NLS
216053AA332Fragmid fragment: (C' terminus) 3x NLS sequences
216054AA375Fragmid fragment: (C' terminus) HIV nuclear export sequence; HA tag
216055AA428Fragmid fragment: (C' terminus) NpNLS; 3xHA; c-mycNLS; c-mycNLS
216056AA006Fragmid fragment: (2A Selection) Blasticidin resistance
216057AA014Fragmid fragment: (2A Selection) Puromycin resistance
216058AA015Fragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFP
216059AA050Fragmid fragment: (2A Selection) violet-excited GFP
216060AA149Fragmid fragment: (2A Selection) Hygromycin resistance
216061AA163Fragmid fragment: (2A Selection) Stop only
216062AA175Fragmid fragment: (2A Selection) EGFP
216063AA176Fragmid fragment: (2A Selection) mCherry
216064AA266Fragmid fragment: (2A Selection) TagRFP
216065AA267Fragmid fragment: (2A Selection) TagRFP657
216066AA276Fragmid fragment: (2A Selection) Neomycin resistance
216067AA333Fragmid fragment: (2A Selection) Stop; IRES2; Start; PuroR; Stop
216068AA334Fragmid fragment: (2A Selection) Stop; IRES2; Start; BlastR; Stop
216069AA335Fragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; Stop
216070AA406Fragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistance
216071AA303Fragmid fragment: (guide cassette) guide expression
216072AA304Fragmid fragment: (guide cassette) guide expression
216073AA269Fragmid fragment: (CROPseq) WPRE; 3'LTR; guide expression bGHpolyA
216074AA271Fragmid fragment: (CROPseq) guide expression; UMI-compatible
216075pRDA_512RSV-driven, lentiGuide-based destination vector
216076pRDA_575Piggybac, constitutive expression destination vector
216077pRDA_722CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)
216078pRDA_789CROPseq, CMV-driven destination vector
216079pRDA_791Minimal plasmid; contains only ori and ampR, destination vector
216080pRDA_889ITRs from AAV2, destination vector
216081pRDB_051for stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vector
216082pRDB_052for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion, destination vector
216083pRDB_053for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector
216084pRDA_792for adding plate and well barcodes via 6-9 and 9-10 modules, destination vector
216085pRDA_832Cas9 [Sp] knockout targeting CD47, positive control
216086pRDA_833Cas9 [Sp] knockout targeting CD47, positive control
216087pRDA_834Cas9 [Sp] knockout targeting CD59, positive control
216088pRDA_836Cas9 [Sp] knockout targeting CD55, positive control
216089pRDB_118Cas9 [Sp] CRISPRa targeting CD47, positive control
216090pRDB_156Cas9 [Sp] CRISPRa targeting CD274, positive control
216091pRDB_161Cas9 [Sp] CRISPRi targeting CD81, positive control
216092pRDB_162Cas9 [Sp] CRISPRi targeting CD47, positive control
216093pRDB_166Cas9 [Sp] CRISPRi targeting CD55, positive control
216094pRDA_936Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control
216095pRDB_131Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control
216096pRDB_054All-in-one, knockout, delivers Cas12a & gRNA
216097pRDA_734All-in-one, knockout, delivers Cas9 & gRNA
216098pRDA_816CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)
216099pMV_AA090Knockout, EF1a-driven Cas12a (Cas only)
216100pRDA_831CRISPRa, EF1a-driven dCas9-VP64 (Cas only)
216101pRDA_662CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)
216102pRDB_181CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)
216103pRDB_182CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)
216104pMV_AA088Knockout, EF1a-driven Cas9 (Cas only)
216105pMV_AA089gRNA expression for Cas12a (guide only)
216106pMV_AA091CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)
216107pMV_AA050gDNA expression for Cas9 with ChenF+E trRNA (guide only)

Antibodies from Article