A more efficient method to generate integration-free human iPS cells.
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S
Nat Methods. 2011 May;8(5):409-12 PubMed Article
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S
Nat Methods. 2011 May;8(5):409-12 PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 27076 | pCXLE-hOCT3/4 | Non-integrating (episomal) expression of human OCT3/4 |
| 27077 | pCXLE-hOCT3/4-shp53-F | Integration-free (episomal) expression of human OCT3/4 and shRNA against p53 |
| 27078 | pCXLE-hSK | Integration-free (episomal) expression of human SOX2 and KLF4 |
| 27079 | pCXLE-hMLN | Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG |
| 27080 | pCXLE-hUL | Integration-free (episomal) expression of human L-MYC and LIN28 |
| 27081 | pCXLE-Fbx15-cont2 | |
| 27082 | pCXLE-EGFP | Integration-free (episomal) expression of EGFP |