Addgene - pCXLE-hUL Plasmid Data

Plasmid 27080: pCXLE-hUL

Gene/insert name:
L-MYC
Alt name:
LIN28
Insert size:
1871
Species:
H. sapiens (human)
Entrez Gene:
LIN28A (CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
MYCL1 (RP1-118J21.4, LMYC, MYCL, bHLHe38)
Vector backbone:
pCXLE
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
10180
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F List of Sequencing Primers
3' sequencing primer:
WPRE-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pCEP4 is from Invitrogen. CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. Mutation(s) made by Provider lab: Two genes are connected with the foot-and-mouth disease virus 2A self-cleaving sequence.
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A more efficient method to generate integration-free human iPS cells. Okita et al (Nat Methods. 2011 Apr 3. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27080" in your Materials and Methods section.