An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.
Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA
PLoS One. 2011 . 6(3):e17896. PubMed Article
Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA
PLoS One. 2011 . 6(3):e17896. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 54605 | mCerulean3-C1 | Localization: C1 Cloning Vector, Excitation: 433, Emission: 475 |
| 54666 | mCerulean-pBAD | |
| 54721 | mCerulean3-Lifeact-7 | Localization: Actin, Excitation: 433, Emission: 475 |
| 54730 | mCerulean3-N1 | Localization: N1 Cloning Vector, Excitation: 433, Emission: 475 |
| 55399 | mCerulean3-Actin-7 | Localization: Actin, Excitation: 433, Emission: 475 |
| 55400 | mCerulean3-Actin-C-18 | Localization: Actin, Excitation: 433, Emission: 475 |
| 55401 | mCerulean3-Alpha-5-Integrin-12 | Localization: Focal Adhesions, Excitation: 433, Emission: 475 |
| 55403 | mCerulean3-CAF1-C-10 | Localization: Chromatin Assembly Factor 1, Excitation: 433, Emission: 475 |
| 55404 | mCerulean3-Caveolin-C-10 | Localization: Membrane, Excitation: 433, Emission: 475 |
| 55405 | mCerulean3-CD36-C-10 | Localization: Membrane, Excitation: 433, Emission: 475 |
| 55406 | mCerulean3-CDC42-C-10 | Localization: Membrane, Excitation: 433, Emission: 475 |
| 55407 | mCerulean3-CEBPA237-C-4 | Localization: Nucleus, Excitation: 433, Emission: 475 |
| 55408 | mCerulean3-Clathrin-15 | Localization: Clathrin Vesicles, Excitation: 433, Emission: 475 |
| 55409 | mCerulean3-C-Src-7 | Localization: Membrane/Vesicles, Excitation: 433, Emission: 475 |
| 55410 | mCerulean3-Cx26-7 | Localization: Gap Junctions, Excitation: 433, Emission: 475 |
| 55411 | mCerulean3-Cx43-7 | Localization: Gap Junctions, Excitation: 433, Emission: 475 |
| 55412 | mCerulean3-CytERM-N-17 | Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475 |
| 55413 | mCerulean3-Dectin1A-C-10 | Localization: Membrane, Excitation: 433, Emission: 475 |
| 55414 | mCerulean3-Dectin1A-N-10 | Localization: Membrane, Excitation: 433, Emission: 475 |
| 55415 | mCerulean3-EB1-C-18 | Localization: MT End Binding Protein, Excitation: 433, Emission: 475 |
| 55416 | mCerulean3-EB3-7 | Localization: MT End Binding Protein, Excitation: 433, Emission: 475 |
| 55417 | mCerulean3-EB3-C-20 | Localization: MT End Binding Protein, Excitation: 433, Emission: 475 |
| 55418 | mCerulean3-ER-5 | Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475 |
| 55419 | mCerulean3-Fibrillarin-7 | Localization: Nucleoli, Excitation: 433, Emission: 475 |
| 55420 | mCerulean3-Golgi-7 | Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475 |
| 55421 | mCerulean3-H2B-6 | Localization: Nucleus/Histones, Excitation: 433, Emission: 475 |
| 55422 | mCerulean3-H2B-C-10 | Localization: Nucleus/Histones, Excitation: 433, Emission: 475 |
| 55423 | mCerulean3-Integrin-Beta1-N-18 | Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475 |
| 55424 | mCerulean3-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475 |
| 55425 | mCerulean3-LaminB1-10 | Localization: Nuclear Envelope, Excitation: 433, Emission: 475 |
| 55426 | mCerulean3-Lasp1-N-10 | Localization: Focal Adhesions, Excitation: 433, Emission: 475 |
| 55427 | mCerulean3-LC-Myosin-C-10 | Localization: Light Chain Myosin, Excitation: 433, Emission: 475 |
| 55428 | mCerulean3-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 433, Emission: 475 |
| 55429 | mCerulean3-MannII-N-10 | Localization: Mannosidase/Golgi, Excitation: 433, Emission: 475 |
| 55431 | mCerulean3-MAPTau-C-10 | Localization: Microtubules, Excitation: 433, Emission: 475 |
| 55432 | mCerulean3-MCHR1-N-10 | Localization: Cilium, Excitation: 433, Emission: 475 |
| 55433 | mCerulean3-Mito-7 | Localization: Mitochondria, Excitation: 433, Emission: 475 |
| 55434 | mCerulean3-Myotilin-C-14 | Localization: Cytoskeleton, Excitation: 433, Emission: 475 |
| 55435 | mCerulean3-Palladin-C-7 | Localization: Cytoskeleton, Excitation: 433, Emission: 475 |
| 55436 | mCerulean3-Paxillin-22 | Localization: Focal Adhesions, Excitation: 433, Emission: 475 |
| 55437 | mCerulean3-PCNA-19-SV40NLS-4 | Localization: Nucleus, Excitation: 433, Emission: 475 |
| 55438 | mCerulean3-Plastin-C-10 | Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475 |
| 55439 | mCerulean3-PMP-N-10 | Localization: Peroxisomes, Excitation: 433, Emission: 475 |
| 55440 | mCerulean3-Profilin-10 | Localization: Cytoskeleton, Excitation: 433, Emission: 475 |
| 55441 | mCerulean3-PTK2-C-10 | Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475 |
| 55442 | mCerulean3-PTK2-N-10 | Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475 |
| 55443 | mCerulean3-Rab4a-7 | Localization: Ras GTPase, Excitation: 433, Emission: 475 |
| 55444 | mCerulean3-Rab5a-7 | Localization: Ras GTPase, Excitation: 433, Emission: 475 |
| 55445 | mCerulean3-SiT-N-15 | Localization: Golgi TGN, Excitation: 433, Emission: 475 |
| 55446 | mCerulean3-SSTR3-N-17 | Localization: Cilium, Excitation: 433, Emission: 475 |
| 55447 | mCerulean3-Talin-18 | Localization: Focal Adhesions, Excitation: 433, Emission: 475 |
| 55448 | mCerulean3-Talin-N-10 | Localization: Focal Adhesions, Excitation: 433, Emission: 475 |
| 55449 | mCerulean3-TOMM20-N-10 | Localization: Outer Mitochondrial Membrane, Excitation: 433, Emission: 475 |
| 55450 | mCerulean3-Tubulin-C-18 | Localization: Microtubules, Excitation: 433, Emission: 475 |
| 55452 | mCerulean3-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 433, Emission: 475 |
| 55453 | mCerulean3-Vinculin-23 | Localization: Focal Adhesions, Excitation: 433, Emission: 475 |
| 55454 | mCerulean3-Zyxin-6 | Localization: Focal Adhesions, Excitation: 433, Emission: 475 |